pQCXIN X2/pTER shEGFP#1 (w491-1)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19167||Plasmid sent as bacteria in agar stab||1||$65||Add to Cart|
- Backbone size w/o insert (bp) 7721
Vector typeRetroviral, RNAi
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Growth instructionsTOP10F', 37oC
Copy numberHigh Copy
Gene/Insert nameEnhanced green fluorescent protein shRNA #2
Alt nameshGFP #2
Insert Size (bp)65
- Cloning method Restriction Enzyme
- 5′ cloning site Bgl II (destroyed during cloning)
- 3′ cloning site Hind III (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
shRNa oligo seq 5'-CGAAGCAGCACGACTTCTTCTTCAAGAGAGAAGAAGTCGTGCTGCTTCTTTTT
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pQCXIN X2/pTER shEGFP#1 (w491-1) was a gift from Eric Campeau (Addgene plasmid # 19167)
For your References section:A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.