Addgene - pENTR4-myc-nuc (w556-1) Plasmid Data

Plasmid 20026: pENTR4-myc-nuc (w556-1)

Gene/insert name:
no insert
Fusion protein or tag:
NLS
Terminal:
C terminal on backbone
Fusion protein or tag:
Myc
Terminal:
C terminal on backbone
Vector backbone:
pENTR4
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression ; Gateway Entry vector
Backbone size (bp):
2449
Cloning method:
Gateway Cloning
Cloning site 5':
Nco I
Site destroyed during cloning:
No
Cloning site 3':
Bam HI
Site destroyed during cloning:
Yes
5' sequencing primer:
ENTRforw List of Sequencing Primers
3' sequencing primer:
ENTRrev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Nuc-myc derived from pCMV/myc/nuc (Invitrogen)
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20026" in your Materials and Methods section.