1
Plasmid 17423: pENTR4-FLAG (w210-2)
  • Gateway entry vector for N-term FLAG tag

  • None

  • FLAG

  • N terminal on backbone

  • pENTR4
    (Search Vector Database)

  • Invitrogen

  • Mammalian Expression ; Entry vector

  • 2333

  • ENTRforw List of Sequencing Primers

  • ENTRrev

  • Kanamycin

  • DH5alpha

  • 37

  • TOP10F', 37oC

  • Low Copy

  • View sequences (3)
  • View map

  • Notes from Addgene (1)

  • Eric Campeau

  • UBMTA

Comments: 

Users may need to follow a low copy DNA prep protocol in order to obtain a sufficient yield of DNA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17423" in your Materials and Methods section.