Addgene - pENTR4-FLAG (w210-2) Plasmid Data

Plasmid 17423: pENTR4-FLAG (w210-2)

Gene/insert name:
None
Fusion protein or tag:
FLAG
Terminal:
N terminal on backbone
Vector backbone:
pENTR4
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression ; Entry vector
Backbone size (bp):
2333
5' sequencing primer:
ENTRforw List of Sequencing Primers
3' sequencing primer:
ENTRrev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
Low Copy
Sequence: View sequences (3)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Comments:

Users may need to follow a low copy DNA prep protocol in order to obtain a sufficient yield of DNA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17423" in your Materials and Methods section.