- Backbone size (bp) 2333
Vector typeMammalian Expression ; Entry vector
Growth in Bacteria
Growth instructionsTOP10F', 37oC
Copy numberLow Copy
/ Fusion Protein
- FLAG (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer ENTRforw
- 3′ sequencing primer ENTRrev (Common Sequencing Primers)
Users may need to follow a low copy DNA prep protocol in order to obtain a sufficient yield of DNA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pENTR4-FLAG (w210-2) was a gift from Eric Campeau (Addgene plasmid # 17423)
For your References section:A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.