1
Plasmid 17455: pL106P-LUC-ENTR1A(w451-1)
  • Gateway entry vector, Degron N-term Luciferase

  • FKBP-Luciferase fusion

  • L106P-Luciferase fusion

  • 1982

  • firefly

  • AAA89088

  • MTOR (FRAP, FRAP1, FRAP2, RAFT1, RAPT1)

  • FKBP12DD

  • N terminal on insert

  • pENTR1A
    (Search Vector Database)

  • Invitrogen

  • Luciferase ; Entry vector

  • 2280

  • BamHI

  • No

  • Xho I

  • No

  • ENTRforw List of Sequencing Primers

  • ENTRrev

  • Kanamycin

  • DH5alpha

  • 37

  • TOP10F', 37oC

  • High Copy

  • L106P-LUC was generated in the Wandless lab (pBMN FKBP L106P-PpLuc IRES Blasticidin plasmid)

  • View sequences (2)
  • View map

  • Eric Campeau

  • UBMTA
    Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17455" in your Materials and Methods section.