Addgene - pL106P-LUC-ENTR1A(w451-1) Plasmid Data

Plasmid 17455: pL106P-LUC-ENTR1A(w451-1)

Gene/insert name:
FKBP-Luciferase fusion
Alt name:
L106P-Luciferase fusion
Insert size:
1982
Species:
firefly
GenBank ID:
AAA89088
Entrez Gene:
MTOR (FRAP, FRAP1, FRAP2, RAFT1, RAPT1)
Fusion protein or tag:
FKBP12DD
Terminal:
N terminal on insert
Vector backbone:
pENTR1A
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Luciferase ; Entry vector
Backbone size w/o insert (bp):
2280
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
Xho I
Site destroyed during cloning:
No
5' sequencing primer:
ENTRforw List of Sequencing Primers
3' sequencing primer:
ENTRrev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
L106P-LUC was generated in the Wandless lab (pBMN FKBP L106P-PpLuc IRES Blasticidin plasmid)
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17455" in your Materials and Methods section.