Addgene - pENTR4-FKBP12DD N-term B (w385-4) Plasmid Data

Plasmid 17415: pENTR4-FKBP12DD N-term B (w385-4)

Gene/insert name:
None
Fusion protein or tag:
FKBP12DD (L106P)
Terminal:
N terminal on backbone
Vector backbone:
pENTR4
(Search Vector Database)
Vector type:
Mammalian Expression ; Entry vector
Backbone size (bp):
2607
5' sequencing primer:
ENTRforw List of Sequencing Primers
3' sequencing primer:
ENTRrev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17415" in your Materials and Methods section.