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Plasmid 17430: pEF-ENTR-LacZ (710-1)
  • Gateway entry vector with EF1-a promoter, V5-His C-term, Lac Z

  • LacZ

  • beta-galactosidase

  • 3056

  • E.coli

  • V5

  • C terminal on backbone

  • His

  • C terminal on backbone

  • pENTR1A
    (Search Vector Database)

  • Mammalian Expression ; Entry vector

  • 3789

  • Ngo MIV

  • No

  • Bst BI

  • No

  • ENTRforw List of Sequencing Primers

  • ENTRrev

  • Kanamycin

  • DH5alpha

  • 37

  • TOP10F', 37oC

  • High Copy

  • View sequences (2)
  • View map

  • Eric Campeau

  • UBMTA

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17430" in your Materials and Methods section.