Addgene - pEF-ENTR-LacZ (710-1) Plasmid Data

Plasmid 17430: pEF-ENTR-LacZ (710-1)

Gene/insert name:
LacZ
Alt name:
beta-galactosidase
Insert size:
3056
Species:
E.coli
Fusion protein or tag:
V5
Terminal:
C terminal on backbone
Fusion protein or tag:
His
Terminal:
C terminal on backbone
Vector backbone:
pENTR1A
(Search Vector Database)
Vector type:
Mammalian Expression ; Entry vector
Backbone size w/o insert (bp):
3789
Cloning site 5':
Ngo MIV
Site destroyed during cloning:
No
Cloning site 3':
Bst BI
Site destroyed during cloning:
No
5' sequencing primer:
ENTRforw List of Sequencing Primers
3' sequencing primer:
ENTRrev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17430" in your Materials and Methods section.