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Plasmid 19360: pLenti PGK V5-LUC Puro (w543-1)
  • 3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Puro

  • Firefly Luciferase

  • LUC

  • 1721

  • V5

  • N terminal on backbone

  • p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
    (Search Vector Database)

  • Mammalian Expression, Lentiviral, Luciferase

  • 8010

  • Bam HI

  • No

  • Xba I

  • No

  • PGKforw List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • Stbl3, 37oC

  • High Copy

  • Puromycin

  • View sequences (2)
  • View map

  • Notes from Addgene (1)

  • Eric Campeau

  • UBMTA
    Luciferase Limited Use Label License
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

Luciferase cDNA was derived from the pGL3 plasmid (Stratagene)

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19360" in your Materials and Methods section.