pLenti X1 Puro DEST (694-6)
Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size (bp) 9014
Vector typeMammalian Expression, Lentiviral, RNAi ; Destination vector
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Chloramphenicol
Growth instructionsDB3.1 or ccDB survival, 37oC
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
Please note that there are several mismatches in sequence between depositor's sequence and Addgene's quality control sequence for this plasmid. These mismatches are in backbone regions and should not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti X1 Puro DEST (694-6) was a gift from Eric Campeau (Addgene plasmid # 17297)
For your References section:A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.