Addgene - pLenti X1 Puro DEST (694-6) Plasmid Data

Plasmid 17297: pLenti X1 Puro DEST (694-6)

Gene/insert name:
None
Vector backbone:
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi ; Destination vector
Backbone size (bp):
9014
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin and Chloramphenicol
Growth strain(s)
DB3.1
Growth temperature (℃):
37
Growth instructions:
DB3.1 or ccDB survival, 37oC
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17297" in your Materials and Methods section.