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Plasmid 17297: pLenti X1 Puro DEST (694-6)
  • 3rd gen lentiviral promoter-less Gateway destination vector, Puro

  • None

  • p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
    (Search Vector Database)

  • Mammalian Expression, Lentiviral, RNAi ; Destination vector

  • 9014

  • n/a List of Sequencing Primers

  • Ampicillin and Chloramphenicol

  • DB3.1

  • 37

  • DB3.1 or ccDB survival, 37oC

  • High Copy

  • Puromycin

  • View sequences (3)
  • View map

  • Eric Campeau

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

Please note that there are several mismatches in sequence between depositor's sequence and Addgene's quality control sequence for this plasmid. These mismatches are in backbone regions and should not affect function.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17297" in your Materials and Methods section.