pLenti PGK GFP Blast (w510-5)
Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size w/o insert (bp) 7563
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth instructionsStbl3, 30oC
Copy numberHigh Copy
Gene/Insert nameenhanced green fluorescent protein
Insert Size (bp)719
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site Sal I (not destroyed)
- 5′ sequencing primer PGKforw (Common Sequencing Primers)
Terms and Licenses
There are a few mismatches between Addgene's quality control sequence and depositor's sequence. The mismatches do not affect PGK sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti PGK GFP Blast (w510-5) was a gift from Eric Campeau (Addgene plasmid # 19069)
For your References section:A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.