Addgene - pLenti X2 Zeo/pTER shLUC (w648-1) Plasmid Data

Plasmid 21561: pLenti X2 Zeo/pTER shLUC (w648-1)

Gene/insert name:
Firefly luciferase shRNA
Insert size:
50
Vector backbone:
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
(Search Vector Database)
Vector type:
Lentiviral, RNAi
Backbone size w/o insert (bp):
5596
Cloning site 5':
Bgl II
Site destroyed during cloning:
Yes
Cloning site 3':
Hind III
Site destroyed during cloning:
No
5' sequencing primer:
H1 List of Sequencing Primers
Bacterial resistance(s)
Bleocin (Zeocin)
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3, 37oC, low salt LB
High or low copy:
High Copy
Selectable markers:
Zeocin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21561" in your Materials and Methods section.