pLenti X2 Neo/pTER shLUC (w181-1)
Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size w/o insert (bp) 7919
Vector typeLentiviral, RNAi
Growth in Bacteria
Growth instructionsStbl3, 37oC
Copy numberHigh Copy
Gene/Insert nameFirefly Luciferase shRNA
Insert Size (bp)67
- Cloning method Restriction Enzyme
- 5′ cloning site Bgl II (destroyed during cloning)
- 3′ cloning site Hind III (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
shRNA sequence - 5'-CCTTACGCTGAGTACTTCGAGTGTGCTGTCCTCGAAGTACTCAGCGTAAGTTT
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti X2 Neo/pTER shLUC (w181-1) was a gift from Eric Campeau (Addgene plasmid # 17483)
For your References section:A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.