Addgene - GW1-Peredox-mCherry Plasmid Data

Plasmid 32380: GW1-Peredox-mCherry

Gene/insert name:
Peredox-mCherry
Insert size:
2800
Vector backbone:
GW1
(Search Vector Database)
Backbone manufacturer:
British Biotechnology
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5300
Promoter:
CMV
Cloning site 5':
PspOMI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-f List of Sequencing Primers
3' sequencing primer:
SV40pA-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Gary Yellen
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Hung et al (Cell Metab. 2011 Oct 5;14(4):545-54. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32380" in your Materials and Methods section.