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GW1CMV-Perceval
(Plasmid #21737)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21737 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGW1-CMV
  • Backbone manufacturer
    British Biotech
  • Backbone size w/o insert (bp) 5076
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Perceval (fluorescent ATP:ADP sensor)
  • Species
    Synthetic
  • Insert Size (bp)
    1934
  • Mutation
    improved Kozak sequence
  • Tag / Fusion Protein
    • 5'UTR (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-N (rev) gives 5' third
  • 3′ sequencing primer EGFP-C (fwd) gives middle third
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GW1CMV-Perceval was a gift from Gary Yellen (Addgene plasmid # 21737 ; http://n2t.net/addgene:21737 ; RRID:Addgene_21737)
  • For your References section:

    A genetically encoded fluorescent reporter of ATP:ADP ratio. Berg J, Hung YP, Yellen G. Nat Methods. 2009 Feb . 6(2):161-6. 10.1038/nmeth.1288 PubMed 19122669