Purposegenetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31473||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerBritish Biotechnology
- Backbone size w/o insert (bp) 5300
Vector typeMammalian Expression
Growth in Bacteria
Insert Size (bp)710
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer CACAGCAATCGATCCATG (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GW1-pHRed was a gift from Gary Yellen (Addgene plasmid # 31473 ; http://n2t.net/addgene:31473 ; RRID:Addgene_31473)
For your References section:Imaging Intracellular pH in Live Cells with a Genetically Encoded Red Fluorescent Protein Sensor. Tantama M, Hung YP, Yellen G. J Am Chem Soc. 2011 Jul 6. 133(26):10034-7. 10.1021/ja202902d PubMed 21631110