Addgene - pLenti CMV/TO Puro empty (w175-1) Plasmid Data

Plasmid 17482: pLenti CMV/TO Puro empty (w175-1)

Gene/insert name:
None
Vector backbone:
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size (bp):
8007
Cloning site 5':
Bam HI
Site destroyed during cloning:
No
Cloning site 3':
Xba I
Site destroyed during cloning:
No
5' sequencing primer:
CMVforw List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3, 37oC
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17482" in your Materials and Methods section.