Addgene - pENTR1A-GFP-N2 (FR1) Plasmid Data

Plasmid 19364: pENTR1A-GFP-N2 (FR1)

Gene/insert name:
Enhanced Green Fluorescent Protein
Insert size:
719
Vector backbone:
pENTR1A
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Gateway Entry vector
Backbone size w/o insert (bp):
2348
Cloning method:
Gateway Cloning
Cloning site 5':
Bam HI
Site destroyed during cloning:
Yes
Cloning site 3':
Not I
Site destroyed during cloning:
No
5' sequencing primer:
ENTRforw List of Sequencing Primers
3' sequencing primer:
ENTRrev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
GFP derived from the pEGFP-N1 plasmid (Clontech).
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Comments:

Please note, Addgene's quality control sequence shows that there is a 4bp insertion in the multiple cloning site of this plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19364" in your Materials and Methods section.