|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17576||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepEGFP-C1 (Clontech)
- Backbone size w/o insert (bp) 3417
Vector typeMammalian Expression, Lentiviral
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameRD114 envelope glycoprotein
Alt namehybrid RD114 Env with cytoplasmic tail domain derived from the MLV 4070A Env
Insert Size (bp)2146
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byDr. Yasu Takeuchi
Terms and Licenses
Use packaging construct encoding Tat
see Shimode et al. Virus Genes 45, 393-397 (2012) for details regarding discrepancies in accession number X87829. See Accession number NC_009889.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLTR-RD114A was a gift from Jakob Reiser (Addgene plasmid # 17576 ; http://n2t.net/addgene:17576 ; RRID_Addgene_17576)
For your References section:Transduction of bone-marrow-derived mesenchymal stem cells by using lentivirus vectors pseudotyped with modified RD114 envelope glycoproteins. Zhang XY, La Russa VF, Reiser J. J Virol. 2004 Feb . 78(3):1219-29. 10.1128/JVI.78.3.1219-1229.2004 PubMed 14722277