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PurposepsPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.
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Depositing Labs
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Sequence Information
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Depositor Sequences: None.
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Addgene Sequences: Full (1) Partial (6)
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 63586 | Plasmid sent as bacteria in agar stab | 1 | $65 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepsPAX2
- Total vector size (bp) 10703
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Vector typeintegrase deficient lentiviral packaging vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePackaging plasmid for integrase deficient lentiviral vectors
Resource Information
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Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
psPAX2-D64V was a gift from David Rawlings & Andrew Scharenberg (Addgene plasmid # 63586) -
For your References section:
Tracking genome engineering outcome at individual DNA breakpoints. Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, Scharenberg AM. Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648. 10.1038/nmeth.1648 PubMed 21743461