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A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression.
Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
25734pSLIK-VenusLentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Venus fluorescent protein coexpression.
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25735pSLIK-Neo3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Neomycin resistance gene coexpression.
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25736pSLIK-ZeoLentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Zeocin resistance gene coexpression.
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25737pSLIK-Hygro3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.
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25739pSLIK-Venus-TmiR-LucControl lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Venus fluorescent protein coexpression.
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25740pSLIK-Venus-TmiR-G12Lentiviral vector with Tet-based inducible expression of mouse G alpha 12 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.
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25741pSLIK-Venus-TmiR-G13Lentiviral vector with Tet-based inducible expression of mouse G alpha 13 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.
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25742pSLIK-Venus-TmiR-G13-G12Lentiviral vector with Tet-based inducible expression of both mouse G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus fluorescent protein coexpression.
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25743pSLIK-Neo-TmiR-LucControl lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Neomycin resistance gene coexpression.
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25744pSLIK-Neo-TmiR-Gb2Control lentiviral vector with Tet-based inducible expression of mouse G beta 2 miR30-based shRNA, constitutive Neomycin resistance gene coexpression.
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25745pSLIK-Neo-TGmiR-LucControl lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.
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25746pSLIK-Neo-TGmiR-Gb2Control lentiviral vector with Tet-based inducible expression of mouse G beta 2 miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.
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25747pEN_hUmiRc2Entry vector for cloning miR30-based shRNA driven by human U6 promoter.
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25748pEN_TmiRc3Entry vector for cloning miR30-based shRNA driven by TRE promoter.
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25749pEN_TGmiRc3Entry vector for cloning miR30-based shRNA driven by TRE promoter with GFP coexpression.
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25750pEN_TRmiRc2Entry vector for cloning miR30-based shRNA driven by TRE promoter with dsRed2 coexpression.
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25751pEN_TmcsEntry vector with TRE promoter followed by multiple cloning site.
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25752pEN_TTmiRc2Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter.
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25753pEN_TTGmiRc2Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter with GFP coexpression.
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25754pEN_TTRmiRc2Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter with dsRed2 coexpression.
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25755pEN_TTmcsEntry vector with TRE-tight promoter followed by multiple cloning site.
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25756pEN_hU6miR-Luc-AEntry vector with human U6 promoter driving control Luciferase miR30-based shRNA.
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25757pEN_hU6miR-G12-EEntry vector with human U6 promoter driving mouse G alpha 12 miR30-based shRNA.
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25758pEN_hU6miR-G13-LEntry vector with human U6 promoter driving mouse G alpha 13 miR30-based shRNA.
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25759pEN_hU6miR-Gb2-KEntry vector with human U6 promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.
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25760pEN_TmiR_Luc-AEntry vector withTRE promoter driving control Luciferase miR30-based shRNA.
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25761pEN_TmiR_G12-EEntry vector with TRE promoter driving mouse G alpha 12 miR30-based shRNA.
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25762pEN_TmiR_G13-LEntry vector with TRE promoter driving mouse G alpha 13 miR30-based shRNA.
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25763pEN_TmiR_G13-L_G12-EEntry vector with TRE promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.
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25764pEN_TmiR_Gb2-KEntry vector with TRE promoter driving mouse G beta 2 miR30-based shRNA.
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25765pEN_TGmiR_Luc-AEntry vector withTRE promoter driving control Luciferase miR30-based shRNA with GFP coexpression.
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25766pEN_TGmiR_Gb2-KEntry vector withTRE promoter driving mouse G beta 2 miR30-based shRNA with GFP coexpression.
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25767pEN_TRE_GFPEntry vector with TRE promoter driving GFP expression.
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