Addgene - pEN_TmiRc3 Plasmid Data

Plasmid 25748: pEN_TmiRc3

Gene/insert name:
None
Vector backbone:
pENTR1A-Gent
(Search Vector Database)
Backbone manufacturer:
ATCC 10326362
Vector type:
Entry vector
Backbone size (bp):
4608
Cloning site 5':
attL1
Site destroyed during cloning:
No
Cloning site 3':
attL2
Site destroyed during cloning:
No
5' sequencing primer:
pCEP-fwd List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Gentamycin
Growth strain(s)
DB3.1
Growth temperature (℃):
37
Growth instructions:
DB3.1 (ccdB survival)
High or low copy:
Unknown
Person or lab that originally
cloned the gene/insert:
TRE promoter- David Anderson, Caltech; miR-30- Greg Hannon, CSHL
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Iain Fraser
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Comments:

shRNA expression; miR30-based topology; TRE Pmin promoter; Entry vector backbone; +ccdB in parent; Multiple unique sites d/s of TRE

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Shin et al (Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25748" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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