Purpose(Empty Backbone) 3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Neomycin resistance gene coexpression.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25735||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerIain Fraser
- Backbone size (bp) 13286
Vector typeMammalian Expression, Lentiviral, RNAi
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth instructionsDB3.1 (ccdB survival)
- Cloning method Gateway Cloning
- 5′ cloning site attR1 (not destroyed)
- 3′ cloning site attR2 (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hUBCpro-R (CTAAGGCCGAGTCTTATGAGCAG) (Common Sequencing Primers)
Parent lentivector, co-expression of Neomycin selection gene
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSLIK-Neo was a gift from Iain Fraser (Addgene plasmid # 25735 ; http://n2t.net/addgene:25735 ; RRID:Addgene_25735)
For your References section:A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, Rebres R, Roach T, Seaman W, Simon MI, Fraser ID. Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64. 10.1073/pnas.0606179103 PubMed 16945906