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Plasmid 25736: pSLIK-Zeo
  • Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Zeocin resistance gene coexpression.

  • None

  • pSLIK
    (Search Vector Database)

  • Iain Fraser

  • Mammalian Expression, Lentiviral, RNAi

  • 12944

  • Gateway Cloning

  • attR1

  • No

  • attR2

  • No

  • CMV-F, hUBCpro-F List of Sequencing Primers

  • hUBCpro-R

  • Ampicillin and Chloramphenicol

  • DB3.1

  • 37

  • DB3.1 (ccdB survival)

  • Unknown

  • Zeocin

  • rtTA3- Atze Das, University of Amsterdam; ZeoR- Greg Hannon, CSHL; pDS cassette- Invitrogen

  • View sequences (2)
  • View map

  • Notes from Addgene (1)

  • Iain Fraser

  • UBMTA
    Tet Systems Limited Use Label License

Comments: 

Parent lentivector, co-expression of Zeocin selection gene

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Shin et al (Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25736" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only