Addgene - pSLIK-Zeo Plasmid Data

Plasmid 25736: pSLIK-Zeo

Gene/insert name:
None
Vector backbone:
pSLIK
(Search Vector Database)
Backbone manufacturer:
Iain Fraser
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size (bp):
12944
Cloning method:
Gateway Cloning
Cloning site 5':
attR1
Site destroyed during cloning:
No
Cloning site 3':
attR2
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F, hUBCpro-F List of Sequencing Primers
3' sequencing primer:
hUBCpro-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DB3.1
Growth temperature (℃):
37
Growth instructions:
DB3.1 (ccdB survival)
High or low copy:
Unknown
Selectable markers:
Zeocin
Person or lab that originally
cloned the gene/insert:
rtTA3- Atze Das, University of Amsterdam; ZeoR- Greg Hannon, CSHL; pDS cassette- Invitrogen
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Iain Fraser
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Comments:

Parent lentivector, co-expression of Zeocin selection gene

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Shin et al (Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25736" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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