Purpose(Empty Backbone) Entry vector with TRE-tight promoter followed by multiple cloning site.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25755||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerATCC 10326362
- Backbone size (bp) 4218
Vector typeEntry vector
Growth in Bacteria
Growth instructionsDB3.1 (ccdB survival)
- Cloning method Gateway Cloning
- 5′ cloning site attL1 (not destroyed)
- 3′ cloning site attL2 (not destroyed)
- 5′ sequencing primer pCEP-fwd
- 3′ sequencing primer n/a (Common Sequencing Primers)
shRNA expression; miR30-based topology; TRE-TIGHT Pmin promoter + DsRed2 spacer; Entry vector backbone; +ccdB in parent; Spe1 d/s of TRE-TIGHT
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEN_TTmcs was a gift from Iain Fraser (Addgene plasmid # 25755 ; http://n2t.net/addgene:25755 ; RRID:Addgene_25755)
For your References section:A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, Rebres R, Roach T, Seaman W, Simon MI, Fraser ID. Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64. 10.1073/pnas.0606179103 PubMed 16945906