Addgene - pEN_TRmiRc2 Plasmid Data

Plasmid 25750: pEN_TRmiRc2

Gene/insert name:
None
Fusion protein or tag:
dsRed2
Terminal:
N terminal on backbone
Vector backbone:
pENTR1A-Gent
(Search Vector Database)
Backbone manufacturer:
ATCC 10326362
Vector type:
Entry vector
Backbone size (bp):
5309
Cloning site 5':
attL1
Site destroyed during cloning:
No
Cloning site 3':
attL2
Site destroyed during cloning:
No
5' sequencing primer:
pCEP-fwd, DsRed1-C List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Gentamycin
Growth strain(s)
DB3.1
Growth temperature (℃):
37
Growth instructions:
DB3.1 (ccdB survival)
High or low copy:
Unknown
Person or lab that originally
cloned the gene/insert:
TRE promoter- David Anderson, Caltech; miR-30- Greg Hannon, CSHL; dsRed- Tobias Meyer, Stanford
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Iain Fraser
Terms and Licenses:
MTA
Clontech Limited Use Label License
Tet Systems Limited Use Label License

Comments:

shRNA expression; miR30-based topology; TRE Pmin promoter + DsRed2 spacer; Entry vector backbone; +ccdB in parent; Spe1 d/s of TRE

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Shin et al (Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25750" in your Materials and Methods section.