pEN_TTGmiRc2
(Plasmid #25753)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pENTR1A-Gent
  • Backbone manufacturer
    ATCC 10326362
  • Backbone size (bp) 5153
  • Vector type
    Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Growth instructions
    DB3.1 (ccdB survival)
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site attL1 (not destroyed)
  • 3′ cloning site attL2 (not destroyed)
  • 5′ sequencing primer pCEP-fwd, EGFP-C
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

shRNA expression; miR30-based topology; TRE-TIGHT Pmin promoter + GFP spacer; Entry vector backbone; +ccdB in parent; Spe1 d/s of TRE-TIGHT

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEN_TTGmiRc2 was a gift from Iain Fraser (Addgene plasmid # 25753)
  • For your References section:

    A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, Rebres R, Roach T, Seaman W, Simon MI, Fraser ID. Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64. 10.1073/pnas.0606179103 PubMed 16945906