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Plasmid 25753: pEN_TTGmiRc2
  • Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter with GFP coexpression.

  • None

  • GFP

  • N terminal on backbone

  • pENTR1A-Gent
    (Search Vector Database)

  • ATCC 10326362

  • Entry vector

  • 5153

  • attL1

  • No

  • attL2

  • No

  • pCEP-fwd, EGFP-C List of Sequencing Primers

  • n/a

  • Gentamycin

  • DB3.1

  • 37

  • DB3.1 (ccdB survival)

  • Unknown

  • TRE tight promoter- David Anderson, Caltech;

  • View sequences (2)
  • View map

  • Iain Fraser

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

shRNA expression; miR30-based topology; TRE-TIGHT Pmin promoter + GFP spacer; Entry vector backbone; +ccdB in parent; Spe1 d/s of TRE-TIGHT

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Shin et al (Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25753" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only