Purpose(Empty Backbone) Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter with GFP coexpression.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25753||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerATCC 10326362
- Backbone size (bp) 5153
Vector typeEntry vector
Growth in Bacteria
Growth instructionsDB3.1 (ccdB survival)
/ Fusion Protein
- GFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site attL1 (not destroyed)
- 3′ cloning site attL2 (not destroyed)
- 5′ sequencing primer pCEP-fwd, EGFP-C
- 3′ sequencing primer n/a (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byTRE tight promoter- David Anderson, Caltech;
Terms and Licenses
Articles Citing this Plasmid
shRNA expression; miR30-based topology; TRE-TIGHT Pmin promoter + GFP spacer; Entry vector backbone; +ccdB in parent; Spe1 d/s of TRE-TIGHT
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEN_TTGmiRc2 was a gift from Iain Fraser (Addgene plasmid # 25753 ; http://n2t.net/addgene:25753 ; RRID:Addgene_25753)
For your References section:A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, Rebres R, Roach T, Seaman W, Simon MI, Fraser ID. Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64. 10.1073/pnas.0606179103 PubMed 16945906