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(Plasmid #85972)


Item Catalog # Description Quantity Price (USD)
Plasmid 85972 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size (bp) 8985
  • Modifications to backbone
    Cut out PuroR gene with BamHI and AatII and PCR subcloned in HygroR.
  • Vector type
    Lentiviral, RNAi
  • Promoter H1 / TetO
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EZ-Tet-pLKO-Hygro was a gift from Cindy Miranti (Addgene plasmid # 85972 ; ; RRID:Addgene_85972)
  • For your References section:

    A streamlined method for the design and cloning of shRNAs into an optimized Dox-inducible lentiviral vector. Frank SB, Schulz VV, Miranti CK. BMC Biotechnol. 2017 Feb 28;17(1):24. doi: 10.1186/s12896-017-0341-x. 10.1186/s12896-017-0341-x [pii] PubMed 28245848