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pLKO.1 hygro
(Plasmid #24150)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24150 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLKO.1 - TRC cloning vector (no stuffer)
  • Backbone manufacturer
    David Root
  • Backbone size (bp) 7500
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Promoter U6 for shRNA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer LKO1_5
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid is similar to the pLKO.1-TRC cloning vector (www.addgene.org/10878), except the stuffer between AgeI and EcoRI is only about 24 bp (instead of 1.7kb) and the puro gene has been replaced by hygro resistance.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.1 hygro was a gift from Bob Weinberg (Addgene plasmid # 24150)