Purpose(Empty Backbone) 3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24150||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepLKO.1 - TRC cloning vector (no stuffer)
Backbone manufacturerDavid Root
- Backbone size (bp) 7500
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
- Promoter U6 for shRNA
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer LKO1_5
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
This plasmid is similar to the pLKO.1-TRC cloning vector (www.addgene.org/10878), except the stuffer between AgeI and EcoRI is only about 24 bp (instead of 1.7kb) and the puro gene has been replaced by hygro resistance.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLKO.1 hygro was a gift from Bob Weinberg (Addgene plasmid # 24150)