Addgene - pLKO.1 hygro Plasmid Data

Plasmid 24150: pLKO.1 hygro

Gene/insert name:
None
Vector backbone:
pLKO.1 - TRC cloning vector
(Search Vector Database)
Backbone manufacturer:
David Root
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size (bp):
7500
Cloning site 5':
AgeI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
LKO1_5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence: View sequences (2)
Supplemental document:
Addgene's pLKO.1 protocol
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

This plasmid is similar to the pLKO.1-TRC cloning vector (www.addgene.org/10878), except the stuff between AgeI and EcoRI is only about 100 bp (instead of 1.7kb) and the puro gene has been replaced by hygro resistance.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24150" in your Materials and Methods section.