Purpose(Empty Backbone) Tet/Dox inducible shRNA lentivirus with puromycin selection
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85966||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerDmitri Wiederschain
- Backbone size (bp) 10633
Modifications to backboneShrunk stuffer and changed 5' cloning site from AgeI to NheI.
Vector typeLentiviral, RNAi
- Promoter H1 / TetO
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberLow Copy
- 5′ sequencing primer ATTAGTGAACGGATCTCGACGG
- 3′ sequencing primer AACCCAGGGCTGCCTTGG (Common Sequencing Primers)
Modified from Wiederschain et al., Addgene plasmid #21915
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EZ-Tet-pLKO-Puro was a gift from Cindy Miranti (Addgene plasmid # 85966 ; http://n2t.net/addgene:85966 ; RRID:Addgene_85966)
For your References section:A streamlined method for the design and cloning of shRNAs into an optimized Dox-inducible lentiviral vector. Frank SB, Schulz VV, Miranti CK. BMC Biotechnol. 2017 Feb 28;17(1):24. doi: 10.1186/s12896-017-0341-x. 10.1186/s12896-017-0341-x [pii] PubMed 28245848