Addgene - pEN_hUmiRc2 Plasmid Data

Plasmid 25747: pEN_hUmiRc2

Gene/insert name:
None
Vector backbone:
pENTR1A-Gent
(Search Vector Database)
Backbone manufacturer:
ATCC 10326362
Vector type:
Entry vector
Backbone size (bp):
4608
Cloning site 5':
attL1
Site destroyed during cloning:
No
Cloning site 3':
attL2
Site destroyed during cloning:
No
5' sequencing primer:
hU6-F List of Sequencing Primers
Bacterial resistance(s)
Gentamycin
Growth strain(s)
DB3.1
Growth temperature (℃):
37
Growth instructions:
DB3.1 (ccdB survival)
High or low copy:
Unknown
Person or lab that originally
cloned the gene/insert:
U6 promoter and miR-30- Greg Hannon, CSHL
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Iain Fraser
Terms and Licenses:
MTA

Comments:

shRNA expression; miR30-based topology; Human U6 promoter; 27nt U6 leader sequence expressed in transcript; Entry vector backbone; +ccdB in parent;

Addgene sequence shows that there is a T missing in U6 promoter sequence. Depositor is aware of the mismatch and it should not affect plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Shin et al (Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25747" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

25765: pEN_TGmiR_Luc-A