Plasmid 17618: EF.PGK.GFP

• Gene/insert name: None
• Vector backbone: EF.PGK.GFP
  (Search Vector Database)
• Vector type: Mammalian Expression, Lentiviral
• 5' sequencing primer: EF-1a forward
• Bacterial resistance(s) Ampicillin
• Growth strain(s) Stbl3
• Growth temperature (℃): 37
• High or low copy: Unknown
• Selectable markers, other: GFP
• Sequence: View sequences (1)
• Map: View map
• Principal Investigator: Linzhao Cheng
• Terms and Licenses: MTA

Comments: 

In a SIN LV containing the elongation factor 1alpha promoter, we included a PGK promoter that drives expression of GFP as a reporter. Dual-promoter LVs can coexpress multiple transgenes efficiently in a single target cell.

Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.

Article: Lentiviral vectors with two independent internal promoters transfer high-level expression of multiple transgenes to human hematopoietic stem-progenitor cells. Yu et al (Mol Ther. 2003 Jun . 7(6):827-38. PubMed)