Addgene - EF.hICN1.Ubc.GFP Plasmid Data

Plasmid 17626: EF.hICN1.Ubc.GFP

Gene/insert name:
ICN1
Alt name:
intracellular domain of the Notch receptor
Insert size:
2400
Species:
H. sapiens (human)
Entrez Gene:
NOTCH1 (TAN1, hN1)
Vector backbone:
cFUGW
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
11500
5' sequencing primer:
EF-1a Forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers, other:
GFP
Sequence: View sequences (3)
Principal Investigator:
Linzhao Cheng
Terms and Licenses:
MTA

Comments:

Addgene sequence shows that there is an A1902S mutation in Notch1 relative to NCBI sequences. This mutation is not known to affect function.

hICN1 driven by the EF-1a promoter and a GFP reporter driven by the ubiquitin C (Ubc) promoter.

Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding. Yu et al (Stem Cells. 2006 Apr . 24(4):876-88. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17626" in your Materials and Methods section.