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EF.hICN1.Ubc.GFP
(Plasmid #17626)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    cFUGW
  • Backbone size w/o insert (bp) 11500
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    ICN1
  • Alt name
    intracellular domain of the Notch receptor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • Entrez Gene
    NOTCH1 (a.k.a. AOS5, AOVD1, TAN1, hN1)

Cloning Information

Resource Information

Depositor Comments

Addgene sequence shows that there is an A1902S mutation in Notch1 relative to NCBI sequences. This mutation is not known to affect function.

hICN1 driven by the EF-1a promoter and a GFP reporter driven by the ubiquitin C (Ubc) promoter.

Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EF.hICN1.Ubc.GFP was a gift from Linzhao Cheng (Addgene plasmid # 17626)
  • For your References section:

    HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding. Yu X, Alder JK, Chun JH, Friedman AD, Heimfeld S, Cheng L, Civin CI. Stem Cells. 2006 Apr . 24(4):876-88. 10.1634/stemcells.2005-0598 PubMed 16513761