Addgene - EF.hICN1.CMV.GFP Plasmid Data

Plasmid 17623: EF.hICN1.CMV.GFP

Gene/insert name:
ICN1
Alt name:
intracellular domain of the Notch receptor
Insert size:
2400
Species:
H. sapiens (human)
Entrez Gene:
NOTCH1 (TAN1, hN1)
Vector backbone:
EF.v-CMV.GFP
(Search Vector Database)
Backbone manufacturer:
Linzhao Cheng Lab
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
8200
5' sequencing primer:
EF-1a Forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers, other:
GFP
Sequence: View sequences (2)
Principal Investigator:
Linzhao Cheng
Terms and Licenses:
MTA

Comments:

Addgene sequence shows that there is an A1902S mutation in Notch1 relative to NCBI sequences. This mutation is not known to affect function.

The CMV promoter was inserted immediately upstream of GFP in the EF.GFP LV to make the intermediate LV, EF.V-CMV.GFP. hICN1 was cloned downstream of the EF-1a promoter.

Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding. Yu et al (Stem Cells. 2006 Apr . 24(4):876-88. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17623" in your Materials and Methods section.