Backbone manufacturerI. Verma, Salk
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameflap-Ub promoter-GFP-WRE
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
Plasmid pFUGW was constructed by inserting the following into the multicloning site of HR'CS-G: HIV-1 flap sequence PCR-amplified
from the HIV NLA4.3 genome, the human polyubiquitin promoter-C (gift of L. Thiel, Amgen), the EGFP gene, and the WRE (woodchuck
hepatitis virus posttranscriptional regulatory element) (gift of D. Trono, University of Geneva). Lentiviruses can be produced by cotransfecting the HIV-1 packaging vector Delta8.9 and the VSVG envelope glycoprotein into 293 fibroblasts.
Order of elements: CMV LTR PstI flap PacI Ubiquitin promoter SpeI HindIII PstI SalI XbaI BamHI SmaI KpnI GFP NotI EagI XbaI EcoRI EcoRV HindIII ClaI WRE ClaI SalI XhoI KpnI 3'LTR ApaI PmeI.
Please note that there are 2 gaps upstream of the EGFP between author's provided sequence and Addgene's quality control sequence. These gaps are in the non-coding vector region and should not affect the expression of EGFP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FUGW was a gift from David Baltimore (Addgene plasmid # 14883)
For your References section:Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors. Lois C, Hong EJ, Pease S, Brown EJ, Baltimore D. Science. 2002 Feb 1. 295(5556):868-72. 10.1126/science.1067081 PubMed 11786607
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.