Addgene - pLV-Azurite Plasmid Data

Plasmid 36086: pLV-Azurite

Gene/insert name:
Azurite
Alt name:
enhanced blue fluorescent protein, BFP, eB
Insert size:
734
Species:
Synthetic
Vector backbone:
pLenti-MP2
(Search Vector Database)
Vector type:
Lentiviral
Backbone size (bp):
6782
Cloning method:
Unknown
5' sequencing primer:
CMV fwd List of Sequencing Primers
3' sequencing primer:
WPRE-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
optimal growth in low salt LB (5gm NaCl)
High or low copy:
Unknown
Person or lab that originally
cloned the gene/insert:
The Azurite was obtained from Addgene and is originally from Patrick Daugherty (Mena et al., Nat Biotech 2006).
Sequence: View sequences (3)
Principal Investigator:
Pantelis Tsoulfas
Terms and Licenses:
MTA

Comments:

The backbone is described the Naldini group in Dull et al., 1998 (JVI) and was modified by Anthony Oliva and Jia Pingping at the Miami project.

Azurite is mammalian codon optimized.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36086" in your Materials and Methods section.