Purpose(Empty Backbone) A backbone for adenovirus creation by the AdEasy method, similar to AdTrackCMV (Addgene Plasmid # 16405), except it co-expressed mRFP instead of eGFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50957||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerBert Vogelstein lab (Addgene Plasmid # 16405)
- Backbone size (bp) 8935
Modifications to backbonePCR was used to generate mRFP with XmaI on 5’ and BglII on 3’. This was ligated into AdTrackCMV in place of eGFP using AgeI and BclI.
- Promoter CMV
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer Encap-F (5'-TTTGGGCGTAACCGAGTAAG-3')
- 3′ sequencing primer pShuttle-R (5'-CACAATGCTTCCATCAAACG-3') (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:RedTrackCMV was a gift from James Bamburg (Addgene plasmid # 50957 ; http://n2t.net/addgene:50957 ; RRID:Addgene_50957)