|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18703||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerBert Vogelstein (Addgene plasmid# 16403)
- Backbone size (bp) 9772
Modifications to backboneGateway cassette was inserted into original vector to create the pEZYshuttle Gateway Destination vector
Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Kanamycin
Growth instructionsE.coli strain DB3.1, at 30C
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
pShuttle-CMV Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a shuttle vector for the AdEasy adenoviral vector system.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEZYshuttle was a gift from Yu-Zhu Zhang (Addgene plasmid # 18703 ; http://n2t.net/addgene:18703 ; RRID:Addgene_18703)
For your References section:An in vitro recombination method to convert restriction- and ligation-independent expression vectors. Guo F, Chiang MY, Wang Y, Zhang YZ. Biotechnol J. 2008 Mar . 3(3):370-7. 10.1002/biot.200700170 PubMed 18064608