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pEZYshuttle
(Plasmid #18703)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18703 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pShuttle-CMV
  • Backbone manufacturer
    Bert Vogelstein (Addgene plasmid# 16403)
  • Backbone size (bp) 9772
  • Modifications to backbone
    Gateway cassette was inserted into original vector to create the pEZYshuttle Gateway Destination vector
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DB3.1
  • Growth instructions
    E.coli strain DB3.1, at 30C
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pShuttle-CMV Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a shuttle vector for the AdEasy adenoviral vector system.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEZYshuttle was a gift from Yu-Zhu Zhang (Addgene plasmid # 18703 ; http://n2t.net/addgene:18703 ; RRID:Addgene_18703)
  • For your References section:

    An in vitro recombination method to convert restriction- and ligation-independent expression vectors. Guo F, Chiang MY, Wang Y, Zhang YZ. Biotechnol J. 2008 Mar . 3(3):370-7. 10.1002/biot.200700170 PubMed 18064608