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An in vitro recombination method to convert restriction- and ligation-independent expression vectors.
Guo F, Chiang MY, Wang Y, Zhang YZ
Biotechnol J. 2008 Mar . 3(3):370-7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
18667pBZ-attBtxnl4bcloning vector containing attB flanked TXNL4B useful for recombination based conversion of conventional vectors to Gateway compatible destination vectors
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18668pEZY19pET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria
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18669pEZYHbpKW32 converted Gateway Destination vector for expressing hemoglobin tagged fusion proteins in bacteria
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18671pEZYegfppEGFPC4 converted Gateway Destination vector for expressing EGFP tagged fusion proteins in mammalian cells
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18672pEZY3pcDNA3 converted Gateway Destination vector for mammalian expression
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18700pEZYflagpFlag-CMV-2 converted Gateway Destination vector for expressing Flag tagged fusion proteins in mammalian cells
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18701pEZYmyc-HispcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells
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18703pEZYshuttlepShuttle-CMV converted Gateway Destination vector for AdEasy adenoviral vector system
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18704pEZY45pJG4-5 converted Gateway Destination vector for yeast two-hybrid prey vector
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18705pEZY202pEG202 converted Gateway Destination vector for yeast two-hybrid bait vector
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18707pEZYpcipPIC3.5K converted Gateway Destionation vector for multiple copy integration of gene of interest into Pichia
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