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pEZYmyc-His
(Plasmid #18701)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18701 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1(-)/Myc-His B
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 7755
  • Modifications to backbone
    Gateway cassette was inserted into original vector to create the pEZYmyc-His Gateway Destination vector
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DB3.1
  • Growth instructions
    E.coli strain DB3.1 @ 30C
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc-His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

pcDNA3.1(-)/myc-His B Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an C-terminal myc-His tag.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEZYmyc-His was a gift from Yu-Zhu Zhang (Addgene plasmid # 18701)
  • For your References section:

    An in vitro recombination method to convert restriction- and ligation-independent expression vectors. Guo F, Chiang MY, Wang Y, Zhang YZ. Biotechnol J. 2008 Mar . 3(3):370-7. 10.1002/biot.200700170 PubMed 18064608