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rAAV2-retro helper
(Plasmid #81070)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 81070 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Growth instructions
    Stbl3 or Stbl2 are recommended by depositing lab. This plasmid may be prone to recombination. Please process the stab immediately by streaking on a bacterial plate and testing individual colonies for accuracy. Prepare DNA from a freshly plated colony.
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    rep
  • Species
    adeno-associated virus
  • Insert Size (bp)
    1865
  • Mutation
    Pro2Ala, possibly Asp613Gly

Gene/Insert 2

  • Gene/Insert name
    rAAV2-retro cap
  • Species
    adeno-associated virus
  • Insert Size (bp)
    2237
  • Mutation
    changes made to AAV2 cap: N382D, V708I, insertion of LADQDYTKTA between N587 and R588

Resource Information

Depositor Comments

this vector was generated as a part of the Janelia viral project team in collaboration with David V. Schaffer (Berkeley)

The Janelia Virus Core will be posting their production protocol and general tips for maximizing virus titer on their website: https://www.janelia.org/support-team/virus-services

For a list Addgene viruses packaged with this construct, please visit https://www.addgene.org/viral-service/aav-prep/retrograde/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rAAV2-retro helper was a gift from Alla Karpova & David Schaffer (Addgene plasmid # 81070)
  • For your References section:

    A Designer AAV Variant Permits Efficient Retrograde Access to Projection Neurons. Tervo DG, Hwang BY, Viswanathan S, Gaj T, Lavzin M, Ritola KD, Lindo S, Michael S, Kuleshova E, Ojala D, Huang CC, Gerfen CR, Schiller J, Dudman JT, Hantman AW, Looger LL, Schaffer DV, Karpova AY. Neuron. 2016 Oct 6. pii: S0896-6273(16)30580-3. doi: 10.1016/j.neuron.2016.09.021. 10.1016/j.neuron.2016.09.021 PubMed 27720486