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viral-icon-01.png Viral Service: Retrograde AAV


As part of our Viral Service, Addgene is producing ready-to-use viral vector preparations in a retrograde serotype from the Alla Karpova and David Schaffer laboratories. These viral vector preparations were produced with the rAAV2-retro helper plasmid (Addgene #81070). This serotype permits robust retrograde access and transgene delivery to projection neurons. Specific classes of projection neurons can be targeted by using this serotype to deliver Cre-dependent transgenes in Cre transgenic lines.

As part of our standard viral production, these viral vectors undergo quality control, including AAV titering by SYBR green qPCR, confirmation of the transfer plasmid with PCR, purity assessment by silver staining, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector preparation. In some cases, in vivo testing of these viral preparations was performed by the Karpova lab. Results for our quality control experiments can be found on the material pages for viral catalog items.

Browse Available Retrograde AAV

See our retrograde AAV based on the functional catagories listed below. Narrow down the items available within a category by using the buttons.

  • Controls
  • Recombinases
  • Calcium Sensors
  • Optogenetics
ID Name Promoter Activity Category PI
37825 AAV-CAG-GFP CAG GFP Control Boyden
50465 pAAV-hSyn-EGFP Syn EGFP Control Roth
59462 pAAV-CAG-tdTomato CAG tdTomato Control Boyden
50457 pAAV-hSyn-DIO-EGFP Syn EGFP, Cre-dependent Control Roth
28306 pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent Control Boyden
37120 pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA EF1a Color-flipping switch. Expresses tdTomato in the absence of Cre, but inverts to express EGFP in Cre-positive cells. Control Sabatini
24593 AAV-pgk-Cre PGK Cre expression Recombinases Aebischer
51507 AAV pmSyn1-EBFP-Cre Syn1 BFP-tagged Cre expression Recombinases Zeng
55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate mCherry expression Recombinases Deisseroth
55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a Flpo and physically-separate mCherry expression Recombinases Deisseroth
Calcium Sensors
104488 pGP-AAV-syn-jGCaMP7f-WPRE Syn GCaMP7f Calcium sensor Kim
51085 AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear dTomato Calcium sensor Ting
51083 AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear dTomato, Cre-dependent Calcium sensor Ting
51084 AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate nuclear dTomato Calcium sensor Ting
20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA EF1a Activator, Cre-dependent Optogenetics Deisseroth
20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a Activator, Cre-dependent Optogenetics Deisseroth
28017 AAV-CAG-hChR2-H134R-tdTomato CAG Activator Optogenetics Svoboda
55645 pAAV-hSyn Con/Fon hChR2(H134R)-EYFP Syn Activator, Cre and Flp-dependent Optogenetics Deisseroth
58880 pAAV-Syn-ChR2(H134R)-GFP Syn Activator Optogenetics Boyden
59170 pAAV-Syn-Chronos-GFP Syn Activator Optogenetics Boyden
65014 pAAV-hsyn-Jaws-KGC-GFP-ER2 Syn Inhibitor Optogenetics Boyden
84445 pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] CAG Inhibitor, Cre-dependent Optogenetics Boyden
44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Syn1 Activator, Cre-dependent DREADD Roth
44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor, Cre-dependent DREADD Roth
50474 pAAV-hSyn-hM3D(Gq)-mCherry Syn Activator DREADD Roth
50475 pAAV-hSyn-hM4D(Gi)-mCherry Syn Inhibitor DREADD Roth

Currently, no available plasmids.

Browse all Retrograde AAV

Depositor Comments

The AAV retro helper plasmid was generated as a part of the Janelia Research Campus viral project team in collaboration with David Schaffer (University of California, Berkeley).

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Please let us know what you would like to see available in viral format. Please note this does not guarantee viral service, but lets us know what viruses would be helpful to the scientific community.

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