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viral-icon-01.png Viral Service: Retrograde AAV


As part of our Viral Service, Addgene is producing ready-to-use viral preparations in a retrograde serotype from the Alla Karpova and David Schaffer laboratories. These viral preparations were produced with the rAAV2-retro helper plasmid (Addgene #81070). This serotype permits robust retrograde access and transgene delivery to projection neurons. Specific classes of projection neurons can be targeted by using this serotype to deliver Cre-dependent transgenes in Cre transgenic lines.

As part of our standard viral production, these viruses undergo quality control, including AAV titering by SYBR green qPCR, confirmation of the transfer plasmid with PCR, purity assessment by silver staining, in vitro and (when possible) in vivo testing, and full sequencing of the final viral preparation. In some cases, in vivo testing of these viral preparations was performed by the Karpova lab. Results for our quality control experiments can be found on the material pages for viral catalog items.

Browse Available Retrograde AAV by Category

ID Name Promoter Activity Serotype PI
37825 AAV-CAG-GFP CAG GFP expression rg (retrograde)* Boyden
50465 pAAV-hSyn-EGFP Syn EGFP expression rg (retrograde)* Roth
59462 pAAV-CAG-tdTomato CAG tdTomato expression rg (retrograde)* Boyden
pAAV-hSyn-DIO-EGFP Syn Cre-dependent EGFP expression rg (retrograde)* Roth
pAAV-FLEX-tdTomato CAG Cre-dependent tdTomato expression rg (retrograde)* Boyden
37120 pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA EF1a Cre-dependent color-flipping reporter. Expresses tdTomato in the absence of Cre, but inverts to express EGFP in Cre-positive cells. rg (retrograde)* Sabatini
Site-Specific Recombinases
24593 AAV-pgk-Cre PGK Cre recombinase (codon optimized) rg (retrograde)* Aebischer
51507 AAV pmSyn1-EBFP-Cre Syn1 BFP-tagged Cre recombinase rg (retrograde)* Zeng
55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a Flpo and physically-separate mCherry rg (retrograde)* Deisseroth
55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre recombinase and physically-separate mCherry rg (retrograde)* Deisseroth
Calcium Sensors
51085 AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Syn1 Calcium sensor and physically separate nuclear dTomato rg (retrograde)* Ting
51083 AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a Cre-dependent calcium sensor and physically separate nuclear dTomato rg (retrograde)* Ting
51084 AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn1 Calcium sensor and physically separate nuclear dTomato rg (retrograde)* Ting
20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA EF1a Channelrhodopsin/Excitatory, Cre-dependent rg (retrograde)* Deisseroth
20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a Channelrhodopsin/Excitatory, Cre-dependent rg (retrograde)* Deisseroth
28017 AAV-CAG-hChR2-H134R-tdTomato CAG Channelrhodopsin/Excitatory rg (retrograde)* Svoboda
pAAV-hSyn Con/Fon hChR2(H134R)-EYFP Syn1 Channelrhodopsin/Excitatory (Cre and Flp-dependent) rg (retrograde)* Deisseroth
58880 pAAV-Syn-ChR2(H134R)-GFP Syn1 Channelrhodopsin/Excitatory rg (retrograde)* Boyden
59170 pAAV-Syn-Chronos-GFP Syn1 Channelrhodopsin/Excitatory rg (retrograde)* Boyden
65014 pAAV-hsyn-Jaws-KGC-GFP-ER2 Syn1 Halorhodopsin/Inhibitory rg (retrograde)* Boyden
84445 pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] CAG Halorhodopsin/Inhibitory (Cre-dependent) rg (retrograde)* Boyden
Chemogenetics, DREADDs
44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Syn1 Chemogenetics/DREADD rg (retrograde)* Roth
44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn1 Chemogenetics/DREADD rg (retrograde)* Roth
50474 pAAV-hSyn-hM3D(Gq)-mCherry Syn1 Chemogenetics/DREADD rg (retrograde)* Roth
50475 pAAV-hSyn-hM4D(Gi)-mCherry Syn1 Chemogenetics/DREADD rg (retrograde)* Roth

*rg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs

Virus associated DNA

Each viral service request will also include virus associated DNA, which is a sample of the purified DNA from the original plasmid that was used to make the virus. In order for Addgene to provide viral services to you, we must ensure that you have the right to use the original plasmid.

Distribution of the original plasmid is important because it is the actual material being referenced in the Material Transfer Agreement (MTA) and its inclusion is what gives you permission to use the plasmid.

Depositor Comments

The AAV retro helper plasmid was generated as a part of the Janelia Research Campus viral project team in collaboration with David Schaffer (University of California, Berkeley).

Don’t See What You’re Looking For?

Please let us know what you would like to see available in viral format using the form below. Please note this does not guarantee viral service, but lets us know what viruses would be helpful to the scientific community.