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viral-icon-01.png Viral Service: Retrograde AAV


CAG-tdTomato.jpg

As part of our Viral Service, Addgene is producing ready-to-use viral vector preparations in a retrograde serotype from the Alla Karpova and David Schaffer laboratories. These viral vector preparations were produced with the rAAV2-retro helper plasmid (Addgene #81070). This serotype permits robust retrograde access and transgene delivery to projection neurons. Specific classes of projection neurons can be targeted by using this serotype to deliver Cre-dependent transgenes in Cre transgenic lines.

As part of our standard viral production, these viral vectors undergo quality control, including AAV titering by SYBR green qPCR, confirmation of the transfer plasmid with PCR, purity assessment by silver staining, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector preparation. In some cases, in vivo testing of these viral preparations was performed by the Karpova lab. Results for our quality control experiments can be found on the material pages for viral catalog items.


Browse Available Retrograde AAV

See our retrograde AAV based on the functional catagories listed below. Narrow down the items available within a category by using the buttons.

  • Controls
  • Recombinases
  • Calcium Sensors
  • Other Biosensors
  • Optogenetics
  • DREADDs
ID Name Promoter Activity Category PI
Controls
37825 AAV-CAG-GFP CAG GFP Control Boyden
51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP, Cre-dependent Control Zeng
50465 pAAV-hSyn-EGFP Syn EGFP Control Roth
114472 pAAV-hSyn-mCherry Syn mCherry Control Deisseroth
50459 pAAV-hSyn-DIO-mCherry Syn mCherry Control Roth
59462 pAAV-CAG-tdTomato CAG tdTomato Control Boyden
50457 pAAV-hSyn-DIO-EGFP Syn EGFP, Cre-dependent Control Roth
55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and Flp-dependent Control Deisseroth
28306 pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent Control Boyden
27056 pAAV-Ef1a-DIO EYFP EF1a EYFP, Cre-dependent Control Deisseroth
114471 pAAV-Ef1a-fDIO mCherry EF1a mCherry, Flp-dependent Control Deisseroth
99130 pAAV-mDlx-NLS-mRuby2 Dlx NLS-mRuby2 Control Gradinaru
83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell
83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Control Fishell
83894 pAAV-hDlx-Flex-dTomato-Fishell_7 Dlx dTomato Control Fishell
112677 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a Color-flipping switch. Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP in Cre-positive cells. Control Harvey
Recombinases
24593 AAV-pgk-Cre PGK Cre expression Recombinases Aebischer
55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate mCherry expression Recombinases Deisseroth
105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn EGFP-tagged Cre expression Recombinases Wilson
107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression with simultaneous dTomato expression Recombinases Larsen
105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn Cre expression Recombinases Wilson
107788 AAV.rTH.PI.Cre.SV40 rTH Cre expression Recombinases Wilson
55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a Flpo and physically-separate mCherry expression Recombinases Deisseroth
55637 pAAV-EF1a-Flpo EF1a Flpo Recombinases Deisseroth
87306 AAV pEF1a-DIO-FLPo-WPRE-hGHpA EF1a Cre-dependent Flpo recombinase Recombinases Zhang
121675 pAAV-EF1a-fDIO-Cre EF1a Flp-dependent Cre recombinase Recombinases Engel, Nectow
50363 AAV phSyn1(S)-DreO-bGHpA Syn Dre expression Recombinases Zeng
Calcium Sensors
51085 AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear dTomato Calcium sensor Ting
51083 AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear dTomato, Cre-dependent Calcium sensor Ting
83899 pAAV-mDlx-GCaMP6f-Fishell-2 mDlx GCaMP6f expression under the control of the mDlx enhancer element Calcium sensor Fishell
51084 AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate nuclear dTomato Calcium sensor Ting
100853 pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 Syn Cre-dependent jRGECO1a Calcium Sensor Kim, GENIE
100854 pAAV.Syn.NES-jRGECO1a.WPRE.SV40 Syn jRGECO1a Calcium Sensor Kim, GENIE
104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn GCaMP7s Calcium sensor Kim
104489 pGP-AAV-syn-jGCaMP7b-WPRE Syn GCaMP7b Calcium sensor Kim
104491 pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn GCaMP7s, Cre-dependent Calcium sensor Kim
104493 pGP-AAV-syn-FLEX-jGCaMP7b-WPRE Syn GCaMP7b, Cre-dependent Calcium sensor Kim
104495
New!
pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG GCaMP7s, Cre-dependent Calcium sensor Kim
104496
pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG GCaMP7f, Cre-dependent Calcium sensor Kim
104488 pGP-AAV-syn-jGCaMP7f-WPRE Syn GCaMP7f Calcium sensor Kim
104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn GCaMP7f, Cre-dependent Calcium sensor Kim
Other Biosensors
106174 pAAV.hSynapsin.SF-iGluSnFR.A184S Syn Tight affinity glutamate sensor Glutamate sensor Looger
113050 pAAV-hSyn-GRAB_DA1h Syn genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h Dopamine sensor Li
123308 pAAV-hSyn-GRAB_NE1m Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m Norepinephrine sensor Li
123309 pAAV-hSyn-GRAB_NE1h Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h Norepinephrine sensor Li
123310 pAAV-hSyn-GRAB_NEmut Syn GRAB-NEmut control norepinephrine sensor (does not respond to NE) Norepinephrine sensor Li
Optogenetics
20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA EF1a Activator, Cre-dependent Optogenetics Deisseroth
20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a Activator, Cre-dependent Optogenetics Deisseroth
26973 pAAV-hSyn-hChR2(H134R)-EYFP Syn Activator Optogenetics Deisseroth
28017 AAV-CAG-hChR2-H134R-tdTomato CAG Activator Optogenetics Svoboda
26975 pAAV-CaMKIIa-hChR2(H134R)-mCherry CamKII Activator Optogenetics Deisseroth
55645 pAAV-hSyn Con/Fon hChR2(H134R)-EYFP Syn Activator, Cre and Flp-dependent Optogenetics Deisseroth
58880 pAAV-Syn-ChR2(H134R)-GFP Syn Activator Optogenetics Boyden
59170 pAAV-Syn-Chronos-GFP Syn Activator Optogenetics Boyden
83898 pAAV-mDlx-ChR2-mCherry-Fishell-3 mDIx Activator Optogenetics Fishell
65014 pAAV-hsyn-Jaws-KGC-GFP-ER2 Syn Inhibitor Optogenetics Boyden
84445 pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] CAG Inhibitor, Cre-dependent Optogenetics Boyden
99039 pAAV-CamKII-ArchT-GFP (PV2527) CamKII Inhibitor Optogenetics Boyden
105677 pAAV_hSyn1-SIO-stGtACR2-FusionRed Syn Inhibitor, Cre-dependent Optogenetics Yizhar
DREADDs
44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Syn Activator, Cre-dependent DREADD Roth
44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor, Cre-dependent DREADD Roth
50474 pAAV-hSyn-hM3D(Gq)-mCherry Syn Activator DREADD Roth
50475 pAAV-hSyn-hM4D(Gi)-mCherry Syn Inhibitor DREADD Roth
83896 pAAV-hDlx-GiDREADD-dTomato-Fishell-5 hDlx Inhibitor DREADD Fishell
83897 pAAV-hDlx-GqDREADD-dTomato-Fishell-4 hDlx Activator DREADD Fishell

Currently, no available plasmids.

Browse all Retrograde AAV



Depositor Comments

The AAV retro helper plasmid was generated as a part of the Janelia Research Campus viral project team in collaboration with David Schaffer (University of California, Berkeley).

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