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Allen Institute for Cell Science Jewel Allen Institute for Cell Science Plasmid Collection


Background

The Allen Institute for Cell Science has produced the first publicly available collection of fluorescently tagged, human, induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited here with Addgene for distribution to the research community.

With these plasmids researchers will be able to generate fluorescently tagged proteins of key cellular structures in human cell lines of their choice. For a full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human Cells on the Addgene Blog.

Schematic for gene editing of Allen Institute for Cell Science stem cells

Allen Institute for Cell Science Plasmids

Find additional cell line information from the Allen Institute for Cell Science data portal:

  • View data and images from the hiPS cell lines
  • Find gRNA sequences used in the creation of the hiPS cell lines

The following plasmids can be used to create cell lines with endogeneously tagged cellular structures.

Addgene ID Plasmid Allen Institute ID Tag Protein Structure
87420 PXN-EGFP AICSDP-1 EGFP Paxillin Matrix Adhesions
87421 TUBA1B-mEGFP AICSDP-4 mEGFP Alpha-tubulin Microtubules
87422 LMNB1-mEGFP AICSDP-10 mEGFP Nuclear laminB1 Nuclear envelope
87423 TOMM20-mEGFP AICSDP-8 mEGFP TOM20 Mitochondria
87424 DSP-mEGFP AICSDP-9 mEGFP Desmoplakin Desmosomes
87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin Actin
87426 SEC61B-mEGFP AICSDP-7 mEGFP Sec61-beta Endoplasmic Reticulum
87427 FBL-mEGFP AICSDP-13 mEGFP Fibrillarin Nucleolus
87428 MYH10-mEGFP AICSDP-24 mEGFP Non-muscle myosin heavy chain IIB Actomyosin bundles
87429 TJP1-mEGFP AICSDP-23 mEGFP Tight junction protein ZO1 Tight junctions
91565 AAVS1-mEGFP AICSDP-35 mEGFP NA Cytoplasm
101781 CETN2-mTagRFP-T AICSDP-22 mTagRFP-T Centrin-2 Centrosome
101783 MAP1LC3A-mEGFP AICSDP-25 mEGFP LC3 Autophagosomes
101784 SLC25A17-mEGFP AICSDP-27 mEGFP Peroxisomal membrane protein PMP34 Peroxisomes
101785 TUBA1B-mTagRFP-T AICSDP-28 mTagRFP-T Alpha tubulin Microtubules
101786 ST6GAL1-mEGFP AICSDP-26 mEGFP Sialyltransferase 1 Golgi

IPSC cell lines can be ordered from the Allen Institute for Cell Science Cell Catalog.

Reference

Roberts B, et al. 2017. Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization. Mol Biol Cell. 28(21):2854-2874. PMID: 28814507

Allen Institute for Cell Science Publications