This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
We have implemented updates to our Transparency and Privacy Policy in light of the new GDPR standards. When logging in or creating a new account, you will be asked to read and acknowledge these policy changes. Additionally, you can find our Transparency and Privacy Policy here.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Allen Institute for Cell Science Jewel Allen Institute for Cell Science Plasmid Collection


The Allen Institute for Cell Science has produced the first publicly available collection of fluorescently tagged, human, induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited here with Addgene for distribution to the research community.

With these plasmids researchers will be able to generate fluorescently tagged proteins of key cellular structures in human cell lines of their choice. For a full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human Cells on the Addgene Blog.

Schematic for gene editing of Allen Institute for Cell Science stem cells

Allen Institute for Cell Science Plasmids

Find additional cell line information from the Allen Institute for Cell Science data portal:

  • View data and images from the hiPS cell lines
  • Find gRNA sequences used in the creation of the hiPS cell lines

The following plasmids can be used to create cell lines with endogeneously tagged cellular structures.

Addgene ID Plasmid Allen Institute ID Tag Protein Structure
87420 PXN-EGFP AICSDP-1 EGFP Paxillin Matrix Adhesions
87421 TUBA1B-mEGFP AICSDP-4 mEGFP Alpha-tubulin Microtubules
87422 LMNB1-mEGFP AICSDP-10 mEGFP Nuclear laminB1 Nuclear envelope
87423 TOMM20-mEGFP AICSDP-8 mEGFP TOM20 Mitochondria
87424 DSP-mEGFP AICSDP-9 mEGFP Desmoplakin Desmosomes
87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin Actin
87426 SEC61B-mEGFP AICSDP-7 mEGFP Sec61-beta Endoplasmic Reticulum
87427 FBL-mEGFP AICSDP-13 mEGFP Fibrillarin Nucleolus
87428 MYH10-mEGFP AICSDP-24 mEGFP Non-muscle myosin heavy chain IIB Actomyosin bundles
87429 TJP1-mEGFP AICSDP-23 mEGFP Tight junction protein ZO1 Tight junctions
91565 AAVS1-mEGFP AICSDP-35 mEGFP NA Cytoplasm
101781 CETN2-mTagRFP-T AICSDP-22 mTagRFP-T Centrin-2 Centrosome
101783 MAP1LC3B-mEGFP AICSDP-25 mEGFP LC3 Autophagosomes
101784 SLC25A17-mEGFP AICSDP-27 mEGFP Peroxisomal membrane protein PMP34 Peroxisomes
101785 TUBA1B-mTagRFP-T AICSDP-28 mTagRFP-T Alpha tubulin Microtubules
101786 ST6GAL1-mEGFP AICSDP-26 mEGFP Sialyltransferase 1 Golgi
109122 NPM1-mEGFP AICSDP-50 mEGFP Nucleophosmin Nucleolus
109120 GJA1-mEGFP AICSDP-43 mEGFP Connexin-43 Gap junction
109121 HIST1H2BJ-mEGFP AICSDP-52 mEGFP Histone H2B type 1-J Nucleus
109119 CTNNB1-mEGFP AICSDP-47 mEGFP Beta catenin Adherens junctions
107579 RAB5A-mEGFP AICSDP-39 mEGFP Ras-related protein Rab-5A Endosomes
107580 AAVS1-mTagRFPT-CAAX AICSDP-42 mTagRFPT-CAAX NA Plasma Membrane

IPSC cell lines can be ordered from the Allen Institute for Cell Science Cell Catalog.


The Allen Institute for Cell Science has several protocol videos available that may be helpful for working with this plasmid collection.


Roberts B, et al. 2017. Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization. Mol Biol Cell. 28(21):2854-2874. PMID: 28814507

Allen Institute for Cell Science Publications