BioBrick^TM Public Agreement Plasmid Collection

About

Addgene is working with the BioBricks Foundation (BBF) to distribute plasmids expressing genetically encoded functions that have been contributed separately by members of the scientific research community under the BioBrick ^TM Public Agreement (BPA).

Donating basic bioengineering knowledge into the public domain using the BPA can help accelerate innovation, collapse timelines, and speed time-to-market of inventive solutions to world problems while fostering ethical use of technology.

Free-to-Use

The BioBrick^TM Public Agreement (BPA) is a free-to-use legal tool that allows individuals, companies, and institutions to make any genetically encoded function that they own or make anew free for others to use.

Once you and/or your company or institution execute the BioBrick^TM User Agreement you are free to use all contributed material made via the BPA. Please note that the original materials may be subject to other agreements that may have been executed by your company or institution.

If you obtain one of these plasmids and subsequently create a derivative plasmid, Addgene and the BBF encourage you to make it free for others to use by completing the BioBrick^TM Contributor Agreement.

Available Plasmids

Addgene has a number of plasmids covered under the BPA. Browse the full list below or view two of the highlighted collections.

BIOFAB Plasmids

A collection of well characterized bacterial transcription and translation initiation elements. These easy-to-use cassettes enable scientists to predictably drive gene expression over a large range of expression.

Boolean Integrase Logic (BIL) gates and amplifiers

A set of permanent amplifying AND, NAND, OR, XOR, NOR, and XNOR gates that can be used to control transcription rates in a variety of organisms.

	ID	Plasmid	Purpose	Vector Type	Backbone	Publication
	44449	AND-gate-V2.0		Bacterial Expression, Synthetic Biology	pSB4A5	Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
	44451	OR gate		Bacterial Expression, Synthetic Biology	pSB4A5	Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
	44452	NOR gate-V2.0		Bacterial Expression, Synthetic Biology	pSB4A5	Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
	44453	XOR gate-V2.0		Bacterial Expression, Synthetic Biology	pSB4A5	Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
	44454	XNOR-gate-V2.0		Bacterial Expression, Synthetic Biology	pSB4A5	Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
	44455	XNOR-gate-V2.0-ASV		Bacterial Expression, Synthetic Biology	pSB4A5	Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
	44456	Dual-recombinase-controller		Synthetic Biology	J64100	Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
	45978	pE-FLP		Bacterial Expression, Synthetic Biology	pKD46	One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
	45980	pOSIP-CH (CamR, HK022)		Bacterial Expression, Synthetic Biology	pOSIP	One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
	45981	pOSIP-CT (CamR, P21)		Bacterial Expression, Synthetic Biology	pOSIP	One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
	45982	pOSIP-KC (KanR, PhiC31)		Bacterial Expression, Synthetic Biology	pOSIP	One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
	45983	pOSIP-KH (KanR, HK022)		Bacterial Expression, Synthetic Biology	pOSIP	One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
	45984	pOSIP-KL (KanR, Lambda)		Bacterial Expression, Synthetic Biology	pOSIP	One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
	45985	pOSIP-KO (KanR, 186)		Bacterial Expression, Synthetic Biology	pOSIP	One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
	45986	pOSIP-KP (KanR, Phi80)		Bacterial Expression, Synthetic Biology	pOSIP	One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
	45987	pOSIP-KT (KanR, P21)		Bacterial Expression, Synthetic Biology	pOSIP	One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
	45988	pOSIP-TH (TetR, HK022)		Bacterial Expression, Synthetic Biology	pOSIP	One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
	45989	pOSIP-TT (TetR, P21)		Bacterial Expression, Synthetic Biology	pOSIP	One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
	47812	pFAB3909	BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD5 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47813	pFAB3910	BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD6 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47814	pFAB3911	BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD7 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47815	pFAB3912	BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD8 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47816	pFAB3913	BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD9 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47817	pFAB3914	BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD10 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47818	pFAB3915	BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD11 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47819	pFAB3916	BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD12 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47820	pFAB3989	BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD13 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47822	pFAB3991	BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD15 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47823	pFAB3992	BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD16 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47824	pFAB3993	BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD17 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47825	pFAB3994	BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD18 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47826	pFAB3995	BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD19 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47827	pFAB3996	BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD20 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47828	pFAB3997	BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD21 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47829	pFAB3998	BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD22 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47830	pFAB3999	BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD23 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47831	pFAB4000	BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD24 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47832	pFAB3665	BIOFAB RFP reporter plasmid for measuring promoter 1 + BCD1 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47833	pFAB3677	BIOFAB RFP reporter plasmid for measuring promoter 2 + BCD1 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47834	pFAB3689	BIOFAB RFP reporter plasmid for measuring promoter 3 + BCD1 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47835	pFAB3701	BIOFAB RFP reporter plasmid for measuring promoter 4 + BCD1 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47836	pFAB3713	BIOFAB RFP reporter plasmid for measuring promoter 5 + BCD1 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47837	pFAB3725	BIOFAB RFP reporter plasmid for measuring promoter 6 + BCD1 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47838	pFAB3737	BIOFAB RFP reporter plasmid for measuring promoter 7 + BCD1 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47839	pFAB3833	BIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47840	pFAB3845	BIOFAB RFP reporter plasmid for measuring promoter 9 + BCD1 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47841	pFAB3857	BIOFAB RFP reporter plasmid for measuring promoter 10 + BCD1 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47842	pFAB3869	BIOFAB RFP reporter plasmid for measuring promoter 11 + BCD1 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47843	pFAB3881	BIOFAB RFP reporter plasmid for measuring promoter 12 + BCD1 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47844	pFAB3893	BIOFAB RFP reporter plasmid for measuring promoter 13 + BCD1 efficiency.	Bacterial Expression, Synthetic Biology	pFAB1781	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47846	pFAB801	BIOFAB reporter plasmid for measuring trp_att_L126 termination efficiency	Bacterial Expression, Synthetic Biology	pFAB763	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47847	pFAB803	BIOFAB reporter plasmid for measuring T7 early termination efficiency	Bacterial Expression, Synthetic Biology	pFAB764	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47848	pFAB804	BIOFAB reporter plasmid for measuring T3 early termination efficiency	Bacterial Expression, Synthetic Biology	pFAB765	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47849	pFAB806	BIOFAB reporter plasmid for measuring lambda tR2 termination efficiency	Bacterial Expression, Synthetic Biology	pFAB766	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47850	pFAB805	BIOFAB reporter plasmid for measuring tonB termination efficiency	Bacterial Expression, Synthetic Biology	pFAB767	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47851	pFAB816	BIOFAB reporter plasmid for measuring ilvGEDA termination efficiency	Bacterial Expression, Synthetic Biology	pFAB768	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47852	pFAB807	BIOFAB reporter plasmid for measuring RNAI termination efficiency	Bacterial Expression, Synthetic Biology	pFAB769	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47853	pFAB808	BIOFAB reporter plasmid for measuring rrnB T1 termination efficiency	Bacterial Expression, Synthetic Biology	pFAB770	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47854	pFAB815	BIOFAB reporter plasmid for measuring rnpB T1 termination efficiency	Bacterial Expression, Synthetic Biology	pFAB771	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47855	pFAB820	BIOFAB reporter plasmid for measuring tetAC termination efficiency	Bacterial Expression, Synthetic Biology	pFAB772	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47856	pFAB822	BIOFAB reporter plasmid for measuring BBa_B1006 termination efficiency	Bacterial Expression, Synthetic Biology	pFAB773	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47857	pFAB1907	BIOFAB reporter plasmid for measuring BBa_B1006 U10 termination efficiency	Bacterial Expression, Synthetic Biology	pFAB774	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47858	pFAB1902	BIOFAB reporter plasmid for measuring M13_central_T_linker_rrnD_T1 termination efficiency	Bacterial Expression, Synthetic Biology	pFAB775	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47859	pFAB1910	BIOFAB reporter plasmid for measuring rpoC termination efficiency	Bacterial Expression, Synthetic Biology	pFAB776	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
	47860	pFAB1935	BIOFAB reporter plasmid for measuring his_T_min_linker_crp_T_min termination efficiency	Bacterial Expression, Synthetic Biology	pFAB777	Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.

Resources & Links

Plasmids in the BIOFAB kit have been described in:
Mutalik, V.K., Guimaraes, J.C., Cambray, G., Lam, C., Christoffersen, M.J., Mai, Q.A., Tran, A.B., Paull, M., Keasling, J.D., Arkin, A.P., Endy, D. Precise and reliable gene expression via standard transcription and translation initiation elements. Nature Methods 2013 10(4), 354-360. PubMed PMID 23474465

Plasmids for BIL gates and amplifiers have been described in:
Bonnet, J., Yin, P., Ortiz, M.E., Subsoontorn, P., Endy, D. Amplifying genetic logic gates. Science 2013, 340(6132), 599-603. PubMed PMID 23539178

Other plasmids have been described in:
St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE. One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. PubMed PMID 24050148

The BioBricks Foundation (BBF) is a public benefit organization that aims to ensure that the engineering of biology is conducted in an open and ethical manner to benefit all people and the planet.