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Biobricks Logo BioBrick TM Public Agreement Plasmid Collection


About

Addgene is working with the BioBricks Foundation (BBF) to distribute plasmids expressing genetically encoded functions that have been contributed separately by members of the scientific research community under the BioBrick TM Public Agreement (BPA).

Donating basic bioengineering knowledge into the public domain using the BPA can help accelerate innovation, collapse timelines, and speed time-to-market of inventive solutions to world problems while fostering ethical use of technology.

Free-to-Use

The BioBrickTM Public Agreement (BPA) is a free-to-use legal tool that allows individuals, companies, and institutions to make any genetically encoded function that they own or make anew free for others to use.

Once you and/or your company or institution execute the BioBrickTM User Agreement you are free to use all contributed material made via the BPA. Please note that the original materials may be subject to other agreements that may have been executed by your company or institution.

If you obtain one of these plasmids and subsequently create a derivative plasmid, Addgene and the BBF encourage you to make it free for others to use by completing the BioBrickTM Contributor Agreement .

Available Plasmids

Addgene has a number of plasmids covered under the BPA. Browse the full list below or view two of the highlighted collections.

A collection of well characterized bacterial transcription and translation initiation elements. These easy-to-use cassettes enable scientists to predictably drive gene expression over a large range of expression.

A set of permanent amplifying AND, NAND, OR, XOR, NOR, and XNOR gates that can be used to control transcription rates in a variety of organisms.

ID Plasmid Purpose Vector Type Backbone Publication
44449AND-gate-V2.0Bacterial Expression, Synthetic BiologypSB4A5Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
44451OR gateBacterial Expression, Synthetic BiologypSB4A5Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
44452NOR gate-V2.0Bacterial Expression, Synthetic BiologypSB4A5Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
44453XOR gate-V2.0Bacterial Expression, Synthetic BiologypSB4A5Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
44454XNOR-gate-V2.0Bacterial Expression, Synthetic BiologypSB4A5Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
44455XNOR-gate-V2.0-ASVBacterial Expression, Synthetic BiologypSB4A5Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
44456Dual-recombinase-controllerSynthetic BiologyJ64100Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
45978pE-FLPBacterial Expression, Synthetic BiologypKD46One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
45980pOSIP-CH (CamR, HK022)Bacterial Expression, Synthetic BiologypOSIPOne-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
45981pOSIP-CT (CamR, P21)Bacterial Expression, Synthetic BiologypOSIPOne-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
45982pOSIP-KC (KanR, PhiC31)Bacterial Expression, Synthetic BiologypOSIPOne-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
45983pOSIP-KH (KanR, HK022)Bacterial Expression, Synthetic BiologypOSIPOne-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
45984pOSIP-KL (KanR, Lambda)Bacterial Expression, Synthetic BiologypOSIPOne-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
45985pOSIP-KO (KanR, 186)Bacterial Expression, Synthetic BiologypOSIPOne-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
45986pOSIP-KP (KanR, Phi80)Bacterial Expression, Synthetic BiologypOSIPOne-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
45987pOSIP-KT (KanR, P21)Bacterial Expression, Synthetic BiologypOSIPOne-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
45988pOSIP-TH (TetR, HK022)Bacterial Expression, Synthetic BiologypOSIPOne-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
45989pOSIP-TT (TetR, P21)Bacterial Expression, Synthetic BiologypOSIPOne-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
47812pFAB3909BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD5 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47813pFAB3910BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD6 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47814pFAB3911BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD7 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47815pFAB3912BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD8 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47816pFAB3913BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD9 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47817pFAB3914BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD10 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47818pFAB3915BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD11 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47819pFAB3916BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD12 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47820pFAB3989BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD13 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47822pFAB3991BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD15 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47823pFAB3992BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD16 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47824pFAB3993BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD17 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47825pFAB3994BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD18 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47826pFAB3995BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD19 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47827pFAB3996BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD20 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47828pFAB3997BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD21 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47829pFAB3998BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD22 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47830pFAB3999BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD23 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47831pFAB4000BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD24 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47832pFAB3665BIOFAB RFP reporter plasmid for measuring promoter 1 + BCD1 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47833pFAB3677BIOFAB RFP reporter plasmid for measuring promoter 2 + BCD1 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47834pFAB3689BIOFAB RFP reporter plasmid for measuring promoter 3 + BCD1 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47835pFAB3701BIOFAB RFP reporter plasmid for measuring promoter 4 + BCD1 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47836pFAB3713BIOFAB RFP reporter plasmid for measuring promoter 5 + BCD1 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47837pFAB3725BIOFAB RFP reporter plasmid for measuring promoter 6 + BCD1 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47838pFAB3737BIOFAB RFP reporter plasmid for measuring promoter 7 + BCD1 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47839pFAB3833BIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47840pFAB3845BIOFAB RFP reporter plasmid for measuring promoter 9 + BCD1 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47841pFAB3857BIOFAB RFP reporter plasmid for measuring promoter 10 + BCD1 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47842pFAB3869BIOFAB RFP reporter plasmid for measuring promoter 11 + BCD1 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47843pFAB3881BIOFAB RFP reporter plasmid for measuring promoter 12 + BCD1 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47844pFAB3893BIOFAB RFP reporter plasmid for measuring promoter 13 + BCD1 efficiency.Bacterial Expression, Synthetic BiologypFAB1781Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47846pFAB801BIOFAB reporter plasmid for measuring trp_att_L126 termination efficiencyBacterial Expression, Synthetic BiologypFAB763Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47847pFAB803BIOFAB reporter plasmid for measuring T7 early termination efficiencyBacterial Expression, Synthetic BiologypFAB764Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47848pFAB804BIOFAB reporter plasmid for measuring T3 early termination efficiencyBacterial Expression, Synthetic BiologypFAB765Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47849pFAB806BIOFAB reporter plasmid for measuring lambda tR2 termination efficiencyBacterial Expression, Synthetic BiologypFAB766Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47850pFAB805BIOFAB reporter plasmid for measuring tonB termination efficiencyBacterial Expression, Synthetic BiologypFAB767Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47851pFAB816BIOFAB reporter plasmid for measuring ilvGEDA termination efficiencyBacterial Expression, Synthetic BiologypFAB768Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47852pFAB807BIOFAB reporter plasmid for measuring RNAI termination efficiencyBacterial Expression, Synthetic BiologypFAB769Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47853pFAB808BIOFAB reporter plasmid for measuring rrnB T1 termination efficiencyBacterial Expression, Synthetic BiologypFAB770Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47854pFAB815BIOFAB reporter plasmid for measuring rnpB T1 termination efficiencyBacterial Expression, Synthetic BiologypFAB771Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47855pFAB820BIOFAB reporter plasmid for measuring tetAC termination efficiencyBacterial Expression, Synthetic BiologypFAB772Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47856pFAB822BIOFAB reporter plasmid for measuring BBa_B1006 termination efficiencyBacterial Expression, Synthetic BiologypFAB773Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47857pFAB1907BIOFAB reporter plasmid for measuring BBa_B1006 U10 termination efficiencyBacterial Expression, Synthetic BiologypFAB774Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47858pFAB1902BIOFAB reporter plasmid for measuring M13_central_T_linker_rrnD_T1 termination efficiencyBacterial Expression, Synthetic BiologypFAB775Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47859pFAB1910BIOFAB reporter plasmid for measuring rpoC termination efficiencyBacterial Expression, Synthetic BiologypFAB776Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
47860pFAB1935BIOFAB reporter plasmid for measuring his_T_min_linker_crp_T_min termination efficiencyBacterial Expression, Synthetic BiologypFAB777Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.

Resources & Links

Plasmids in the BIOFAB kit have been described in:
Mutalik, V.K., Guimaraes, J.C., Cambray, G., Lam, C., Christoffersen, M.J., Mai, Q.A., Tran, A.B., Paull, M., Keasling, J.D., Arkin, A.P., Endy, D. Precise and reliable gene expression via standard transcription and translation initiation elements. Nature Methods 2013 10(4), 354-360. PubMed PMID 23474465

Plasmids for BIL gates and amplifiers have been described in:
Bonnet, J., Yin, P., Ortiz, M.E., Subsoontorn, P., Endy, D. Amplifying genetic logic gates. Science 2013, 340(6132), 599-603. PubMed PMID 23539178

Other plasmids have been described in:
St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE. One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. PubMed PMID 24050148

The BioBricks Foundation (BBF) is a public benefit organization that aims to ensure that the engineering of biology is conducted in an open and ethical manner to benefit all people and the planet.