1

Plasmids for Stem Cell Research

Addgene is a global, non-profit plasmid repository dedicated to making it easier for scientists to share.

Addgene has a wide variety of plasmids for stem cell research. Read Background or Jump to the plasmids.

Background

Quick and easy genome engineering for stem cell research:

Musunuru/Cowan Lab TALEN Kit

Stem cells are proliferative, largely unspecialized cells, with the remarkable potential to develop into many different cell types in the body during development. In the adult, some perform organ maintenance, dividing essentially without limit to replenish other cells throughout life. Stem cells are also distinguished from other cell types in part because when they divide each new cell has the potential either to remain a stem cell or become another type of cell with a more specialized function.

In addition to pluripotent embryonic and multipotent adult (or somatic) stem cells, which have been studied for over 30 years, scientists have more recently developed methods to return fully-differentiated adult somatic cells to a pluripotent stem cell state referred to as induced pluripotent stem cells (iPSCs) via "reprogramming." (View reprogramming plasmids) These iPSCs, much like their ES cell cousins, can give rise to an entire adult organism. Additionally, they can be directly differentiated into specific somatic stem cells or fully differentiated cell types.(View differentiation plasmids)

In some cases it is even possible to directly differentiate one differentiated somatic cell into another via transdifferentiation. (View transdifferentiation plasmids)

Many of the techniques for reprogramming, differentiation and transdifferentiation utilize plasmids carrying one or more genes or their viral product. This guide will help you find plasmids available from Addgene that can be used to do so. For a more general overview of stem cells, their importance and up to date research results visit the NIH Stem Cell information page.

Plasmids used in reprogramming somatic cells into iPS cells

Delivery Method
Species
Depositing PI
Vector
Plasmid(s)
Retrovirus
Human
Kathrin Plath
pMXs
link
Shinya Yamanaka
pMXs
link, link
George Daley
pMIG
link
Juan Belmonte
pMSCV
link
Nobuaki Kikyo
pMXs
link
Retrovirus
Mouse
Shinya Yamanaka
pMXs
link, link
Steven Dowdy
pCX4
link
Hans Schöler
pMX
link
Nobuaki Kikyo
pMXs
link
miR - Retrovirus
Mouse
Duanqing Pei
pMXs
link
Lentivirus
Human
Jose Cibelli
pSIN4
link
James Thomson
pSin / pSin4
link / link
Tim Townes
pDL171
link
Rudolf Jaenisch
FUW-tetO
link, link
Konrad Hochedlinger
FU-tetO
link
James Ellis
PL-Sin
link
Lentivirus
Mouse
Rudolf Jaenisch
FUW-tetO
link, link
Konrad Hochedlinger
pLV-tetO
link
Adenovirus
Mouse
Konrad Hochedlinger
pAd
link
Episomal
Human
James Thomson
pEP4
link
Shinya Yamanaka
pCXLE, pCE
link, link*
Episomal
Mouse
Linzhao Cheng
pCEP4
link
non-viral for in-vitro translation
Human
Derrick Rossi
pcDNA3.3
link
non-viral
Mouse
Keisuke Kaji
pCAG
link

* efficient, integration free generation of iPSCs from cord and peripheral blood

Find gene-specific plasmids (including reporters and RNAi) at the following links: NANOG, OCT4, SOX2, MYC, KLF4, and LIN28.

Direct Differentiation and Transdifferentiation Plasmids

From → To
Delivery Method
Species
Depositing PI
Vector
Plasmid(s)
iPSC → Cardiomyocyte
Lentiviral
Human
Sean Palecek
pSin
link
Fibroblast → Neuron
Lentiviral
Mouse
Marius Wernig
Tet-O-FUW
link
Fibroblast → Neuron
Lentiviral
Human
Jerry Crabtree
pTLemiR
link
Fibroblast → Dopaminergic Neuron
Lentiviral
Human
Malin Parmar
pCCL
link
Fibrblast → Motor Neuron
Retroviral
Human/Mouse
Kevin Eggan
pMXs
link
Fibroblast → Hepatocyte
Retroviral
Mouse
Atsushi Suzuki
pGCDNsam
link
Pancreatic Exocrine → Beta-cell
Adenoviral
Mouse
Douglas Melton
pAd
link
Fibroblast → Sertoli Cell
Lentiviral
Mouse
Rudolf Jaenisch
FUW-tetO
link
Fibroblast → Myocytes
non-viral
Mouse
Andrew Lassar
pCSA
link

Do you have suggestions for other plasmids or categories that should be added to this list?
Email help@addgene.org to help us improve this resource!

Reference Page   |   Top   |   Index