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Timo Otonkoski Lab Plasmids


The Timo Otonkoski Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski T Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001.
An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation Saarimäki-Vire J, Balboa D, Russell MA, Saarikettu J, Kinnunen M, Keskitalo S, Malhi A, Valensisi C, Andrus C, Eurola S, Grym H, Ustinov J, Wartiovaara K, Hawkins RD, Silvennoinen O, Varjosalo M, Morgan NG, Otonkoski T. Cell Reports 2017, 19: 281–294
Otonkoski lab Reporter cell lines plasmids (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
69534CAG-DDdCas9VP192-T2A-EGFP-ires-puroDD-dCas9VP192-T2A-EGFP (Other)Mammalian ExpressionConditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001. DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim. PyCAG Add to Cart
69535pCXLE-dCas9VP192-T2A-EGFP-shP53dCas9-dCas9VP192-GFP-shp53 (Other)CRISPRConditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001. Episomal plasmid encoding dCas9VP192 and p53 shRNA pCXLE Add to Cart
69536pCXLE-dCas9VP192-T2A-EGFPdCas9-dCas9VP192-EGFP (Other)CRISPRConditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001. Episomal plasmid encoding dCas9VP192 pCXLE Add to Cart
69537GG-EBNA-OCT45 concatenated gRNA transcriptional cassettes targeting OCT4CRISPRConditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001. Episomal plasmid encoding 5 gRNAS targeting human OCT4 promoter GGdest Add to Cart
69538GG-destConditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001. Destination plasmid for gRNA concatenation Golden Gate cloning GGdest Add to Cart
78311CAG-Cas9-T2A-EGFP-ires-puroWT SpCas9-T2A-EGFP-ires-puromycin resistance (Other)Mammalian Expression, CRISPRAn Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation Cell Reports 2017, 19: 281–294 Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter. PyCAG Add to Cart
89991pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-PuroSOX2-T2A-2xNLS-TdTomato-F2A-Puro (Homo sapiens)CRISPR Otonkoski lab Reporter cell lines plasmids (unpublished) Donor template for generation of SOX2-ntdTomato reporter cell lines pUC19 Add to Cart
89995CAG-Cas9hCas9 (Other)Mammalian Expression Otonkoski lab Reporter cell lines plasmids (unpublished) Expresses high levels of WT Cas9 for genome editing pCAG Add to Cart