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 Immunology Research Plasmids and Resources


Addgene has a variety of resources for use in immunology research. These resources include:

  • Adaptive or innate immunity constructs
  • Cytokine or chemokine plasmids
  • Genes with a role in cell signaling
  • Plasmid kits

Immune Responses

Mammalian organisms are exposed to millions of potential pathogens daily. Their ability to avoid infection depends on two systems that work in concert, the innate and the adaptive immune systems.

Innate Immunity

adaptive-immunity.png
Antigen-presenting cells activating adaptive immune system.

During the first hours or days of exposure to a pathogen, nonspecific defense mechanisms come into play. This immune response is called innate immunity as it is not specific to a particular pathogen and it is triggered very quickly. This line of defense includes physical barriers such skin and mucosa, anti-microbial chemicals in the blood, and specialized cells that can attack the incoming pathogens.

Adaptive Immunity

In contrast, the adaptive immune response is more complex and specific. During the first days of infection, specialized cells process and present foreign antigens to the adaptive immune system. Once a specific antigen has been recognized, the adaptive immune system uses a complex network of cells and molecules to specifically attack pathogens expressing this antigen. The cells activated during this process will expand and give rise to “memory” cells that can quickly recognize the same antigens if the organism is infected by the pathogen again.

Find Genes of Interest for Innate and Adaptive Immunity

Click on different properties to create a custom filtered list of immunology constructs in Addgene's collection.

Immunity

Cell Type

Cytokine/Chemokine

Presentation

Name Immunity Cell Type Cytokine/Chemokine Presentation Signal Transduction
RIP1 Innate Dendritic cells, monocytes, macrophages, B cells No No Kinase
TAK1 Innate Dendritic cells, monocytes, macrophages, B cells No No Kinase
IRF9 Innate Dendritic cells, monocytes, macrophages, B cells No No Transcription factor
IRF3 Innate Dendritic cells, monocytes, macrophages, B cells No No Transcription factor
TNF Innate Macrophages Cytokine No
IL10 Innate T cells, monocytes Cytokine No
CSF2 (GM-CSF) Innate T cells, monocytes Cytokine No Growth factor
IL36RN Innate Macrophages, keratinocytes Cytokine No
IL1B Innate Macrophages Cytokines No
IL6 Adaptive T cells, macrophages, dendritic cells Cytokine No
TLR4 Innate Dendritic cells, macrophages, B cells No No
CD36 Innate Monocytes, macrophages No No
TLR2 Innate Dendritic cells, macrophages, B cells No No
Myd88 Innate Denditic cells, monocytes, macrophages, B cells No No
NLRP3 Innate Monocytes, B cells No No
CD56 Innate NK cells, NKT cells No No
Neuropilin 1 Innate pDC (plasmacytoid dendritic cells) No No
SLAMF6 Innate NK cells No No
TLR1 Innate Dendritic cells, macrophages, B cells No No
TLR3 Innate Dendritic cells No No
TLR5 Innate Dendritic cells, macrophages, B cells No No
TLR6 Innate Dendritic cells, macrophages, B cells No No
TLR9 Innate Dendritic cells, macrophages, B cells No No
CD14 Innate Dendritic cells, macrophages, monocytes, B cells No No
LY96 (MD-2) Innate Dendritic cells, macrophages, B cells No No
MPO Innate Neutrophils No No
TICAM1(TRIF) Innate Dendritic cells, macrophages, B cells No No
CD14 Innate Monocytes No No
A20 Innate Dendritic cells, macrophages, monocytes, B cells No No
TBK1 Innate Dendritic cells, macrophages, monocytes, B cells No No Kinase
TRAM Innate Dendritic cells, macrophages, monocytes, B cells No No
TGFbR Adaptive & innate Dendritic cells, T cells No No Kinase
IRAK1 Adaptive & innate Dendritic cells, T cells, B cells, macrophages, monocytes No No Kinase
IRAK4 Adaptive & innate Dendritic cells, T cells, B cells, macrophages, monocytes No No Kinase
IRAK2 Adaptive & innate Dendritic cells, T cells, B cells, macrophages, monocytes No No Kinase
Ubc13 Adaptive & innate Dendritic cells, T cells, B cells, macrophages, monocytes No No
TRAF6 Adaptive & innate Dendritic cells, T cells, B cells, macrophages, monocytes No No
TRAF3 Adaptive & innate Dendritic cells, B cells, macrophages, monocytes No No
RAG1 Adaptive T cells, B cells No No Nuclease
Btk Adaptive B cells No No Kinase
Zap70 Adaptive T cells No Yes Kinase
STAT3 Adaptive T cells No No Transcription factor
GATA3 Adaptive T cells No No Transcription factor
FOXP3 Adaptive T cells No No Transcription factor
STAT6 Adaptive T cells, B cells No No Transcription factor
STAT4 Adaptive T cells No No Transcription factor
CD25 Adaptive T cells Cytokine receptor No
IL2 Adaptive T cells Cytokine No
IL17A Adaptive T cells Cytokine No
IL4 Adaptive T cells Cytokine No
CX3CR1 Adaptive T cells, monocytes Chemokine receptor No
CD4 Adaptive T cells, macrophages, B cells No Yes
CD44 Adaptive T cells No No
TIM3 Adaptive T cells No No
CD2 Adaptive T cells No Yes
CD40 (TNFRSF5) Adaptive B cells, monocytes, dendritic cells, macrophages No No
CD8A Adaptive T cells, dendritic cells No Yes
CD8B Adaptive T cells, dendritic cells No Yes
CD3 Adaptive T cells, NKT cells No Yes
B220 Adaptive T cells, B cells No No

Currently, no available constructs with those features.

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cytokine-chemokine.png
Immune cell communication via cytokines/chemokines

Cytokines and Chemokines

Cytokines and chemokines play an important role in cell communication during the course of an immune response against pathogens. Cytokines and chemokines can attract cells to the site of infection, activate them, and then help adaptive immune cells control the immune response. Cytokines and chemokines play a key role in and are messengers between the innate and adaptive immune responses.

Find Genes of Interest for Cytokines or Chemokines

Sort or search to create a custom list of immunology constructs in Addgene's collection.

Name Cell Type Cytokine/Chemokine Growth Factor
CD25 T cells Cytokine receptor No
CSF2 (GM-CSF) Macrophages, T cells, NK cells, endothelial cells Cytokine Yes
CX3CR1 T cells, monocytes Chemokine receptor No
CXCR4 T cells, B cells, monocytes Chemokine receptor No
IL1B Macrophages Cytokine No
IL2 T cells Cytokine No
IL4 T cells Cytokine No
IL6 T cells, macrophages, dendritic cells Cytokine No
IL10 T cells, monocytes Cytokine No
IL17A T cells Cytokine No
IL36RN Macrophages, keratinocytes Cytokine No
TNF Macrophages Cytokine No

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cell-signaling.png
Signal transduction by cytokines and pathogens

Cell Signaling

During an immune response, several intracellular processes are activated leading to the development of a response against pathogens. Kinases, adaptors, and transcription factors trigger the expression of cytokines, chemokines, or molecules involved in cells migration, phagocytosis, and antigen presentation.

Find Genes of Interest for Cell Signaling

Sort or search to create a custom list of immunology constructs in Addgene's collection.

Name Signal Transduction Cell Type
STAT3 Transcription factor T cells
GATA3 Transcription factor T cells
FOXP3 Transcription factor T cells
STAT6 Transcription factor T cells
STAT4 Transcription factor T cells
IRF9 Transcription factor Dendritc cells, monocytes, macrophages, B cells
IRF3 Transcription factor Dendritc cells, monocytes, macrophages, B cells
RAG1 Nuclease T cells, B cells
Btk Kinase B cells
Zap70 Kinase T cells
TGFbR Kinase T cells, dendritic cells
IRAK1 Kinase Dendritic cells, monocytes, macrophages, B cells, T cells
IRAK4 Kinase Dendritic cells, monocytes, macrophages, B cells, T cells
IRAK2 Kinase Dendritic cells, monocytes, macrophages, B cells, T cells
TBK1 Kinase Dendritic cells, monocytes, macrophages, B cells
RIP1 Kinase Dendritic cells, monocytes, macrophages, B cells
TAK1 Kinase Dendritic cells, monocytes, macrophages, B cells

Kits for Immunology Research

  • Human Kinase ORF Kit: 558 distinct human kinases and kinase-related protein Open Reading Frames (ORFs) in pDONR-223 Gateway® Entry vectors from the labs of William Hahn and David Root.

  • Multiple Lentiviral Expression (MuLE) System Kit : A modular and flexible system that can be used to construct complex lentiviruses for modification of mammalian cells with a single viral infection from the lab of Ian Frew.

  • MXS Chaining Kit: From the lab of Pierre Neveu, the MXS-chaining system allows efficient assembly of constructs that are optimized for synthetic biology applications in mammalian systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites and standardized building blocks: 14 different fluorescent proteins, 10 mammalian promoters and enhancers, 3 polyA signals as well as selection cassettes and tools for inducible gene expression.

  • Myristoylated Kinase Kit: Consists of 190 human kinases and kinase-related open reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation sequence and flag-epitope tag to each ORF from the labs of William Hahn and Jean Zhao.

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Do you have suggestions for other plasmids that should be added to this list?

Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!