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Multiple Lentiviral Expression System Kit
(Kit # 1000000060 )

Depositing Lab:   Ian Frew

The Multiple Lentiviral Expression (MuLE) is a modular and flexible system that can be used to construct complex lentiviruses for modification of mammalian cells with a single viral infection.

This kit contains 96 plasmids and is shipped as bacterial glycerol stocks in a 96-well plate format.

Add to Cart
$350 USD + shipping

Available to academics and nonprofits only.

Original Publication

A versatile modular vector system for rapid combinatorial mammalian genetics. Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, Catalano A, Busenhart P, Gonçalves AF, Brandt S, Bode PK, Bode-Lesniewska B, Wild PJ, Frew IJ. J Clin Invest. 2015 Apr 1;125(4):1603-19. doi: 10.1172/JCI79743. Epub 2015 Mar 9. PubMed .

Description

The MuLE (Multiple Lentiviral Expression) system facilitates the simultaneous introduction of multiple genetic alterations into mammalian cells. The MuLE system kit consists of 91 building block Entry vectors and 5 lentiviral Destination vectors to allow simple and rapid MultiSite Gateway recombination-based cloning of complex polycistronic lentiviruses containing up to five different genetic expression elements. These vectors can induce combinatorial constitutive or inducible gene overexpression, knockdown (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion (Cre), together with expression of fluorescent or enzymatic reporters for cellular assays and animal imaging studies. The ability to simultaneously alter multiple genes via infection with a single ecotropic or amphotropic MuLE lentivirus provides significant genetic power and allows high throughput genetic modulation studies in mammalian cells and tissues.

Overview of MuLE vector assembly

Overview of MuLE vector assembly

Schematic overview of a recombination reaction involving four MuLE Entry vectors and a lentiviral Destination vector, allowing combinatorial expression of combinations of the listed genetic elements. A series of vectors with similar genetic elements are also available to allow recombination reactions involving two or three Entry vectors

Kit Documentation

Documents included with shipment:

Icon Multiple Lentiviral Expression System Kit (79.1 KB)

Resources

Download a zip file of MuLE vector sequences in pDRAW32 format or plain text. Please note that the five pMuLE ENTR U6-miR-30 vectors (IDs 62116-62120) do not have a full sequence available.

Icon MuLE vector sequences pDRAW32 (241.7 KB) Icon MuLE vector sequences text files (140.9 KB)

Additional Entry vectors compatible with the MuLE system kit. These plasmids are not included in the kit and must be ordered separately:

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The MuLE system kit was a gift from Ian Frew (Addgene kit # 1000000060).”

For your Reference section:

A versatile modular vector system for rapid combinatorial mammalian genetics. Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, Catalano A, Busenhart P, Gonçalves AF, Brandt S, Bode PK, Bode-Lesniewska B, Wild PJ, Frew IJ. J Clin Invest. 2015 Apr 1;125(4):1603-19. doi: 10.1172/JCI79743. Epub 2015 Mar 9. PubMed .

Multiple Lentiviral Expression (MuLE) system - #1000000060

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Kanamycin
Chloramphenicol and Ampicillin

Inventory

Well Plasmid Resistance
A / 1 pMuLE ENTR MCS L1-R5
Kanamycin
A / 2 pMuLE ENTR MCS L5-L2
Kanamycin
A / 3 pMuLE ENTR MCS R4-R3
Kanamycin
A / 4 pMuLE ENTR MCS L1-L4
Kanamycin
A / 5 pMuLE ENTR MCS L3-L2
Kanamycin
A / 6 pMuLE ENTR MCS L5-L4
Kanamycin
A / 7 pMuLE ENTR CMV L1-R5
Kanamycin
A / 8 pMuLE ENTR CMV L5-L2
Kanamycin
A / 9 pMuLE ENTR CMV L3-L2
Kanamycin
A / 10 pMuLE ENTR CMV R4-R3
Kanamycin
A / 11 pMuLE ENTR CMV loxP L5-L2
Kanamycin
A / 12 pMuLE ENTR SV40 L5-L2
Kanamycin
B / 1 pMuLE ENTR SV40 L3-L2
Kanamycin
B / 2 pMuLE ENTR SFFV L1-R5
Kanamycin
B / 3 pMuLE ENTR SFFV L5-L2
Kanamycin
B / 4 pMuLE ENTR CMV/TO L1-R5
Kanamycin
B / 5 pMuLE ENTR CMV/TO R4-R3
Kanamycin
B / 6 pMuLE ENTR H1 L1-R5
Kanamycin
B / 7 pMuLE ENTR H1 L5-L4
Kanamycin
B / 8 pMuLE ENTR H1 L5-L2
Kanamycin
B / 9 pMuLE ENTR H1 L1-L4
Kanamycin
B / 10 pMuLE ENTR H1 R4-R3
Kanamycin
B / 11 pMuLE ENTR 7SK L1-R5
Kanamycin
B / 12 pMuLE ENTR 7SK L5-L4
Kanamycin
C / 1 pMuLE ENTR 7SK L5-L2
Kanamycin
C / 2 pMuLE ENTR 7SK R4-R3
Kanamycin
C / 3 pMuLE ENTR 7SK L1-L4
Kanamycin
C / 4 pMuLE ENTR U6 L1-R5
Kanamycin
C / 5 pMuLE ENTR U6 L1-L4
Kanamycin
C / 6 pMuLE ENTR U6-miR-30 L1-R5
Kanamycin
C / 7 pMuLE ENTR U6-miR-30 L1-L4
Kanamycin
C / 8 pMuLE ENTR U6-miR-30 L5-L2
Kanamycin
C / 9 pMuLE ENTR U6-miR-30 L5-L4
Kanamycin
C / 10 pMuLE ENTR U6-miR-30 R4-R3
Kanamycin
C / 11 pMuLE ENTR 7SK-miR-30 L1-R5
Kanamycin
C / 12 pMuLE ENTR 7SK-miR-30 L5-L4
Kanamycin
D / 1 pMuLE ENTR 7SK-miR-30 L5-L2
Kanamycin
D / 2 pMuLE ENTR 7SK-miR-30 R4-R3
Kanamycin
D / 3 pMuLE ENTR CMV/TO-miR-30 L1-R5
Kanamycin
D / 4 pMuLE ENTR CMV/TO-miR-30 L5-L2
Kanamycin
D / 5 pMuLE ENTR CMV/TO-miR-30 L1-L4
Kanamycin
D / 6 pMuLE ENTR CMV/TO-miR-30 R4-R3
Kanamycin
D / 7 pMuLE ENTR CMV/TO-miR-30 L5-L4
Kanamycin
D / 8 pMuLE ENTR U6 stuffer sgRNA scaffold L1-R5
Kanamycin
D / 9 pMuLE ENTR U6 stuffer sgRNA scaffold L1-L4
Kanamycin
D / 10 pMuLE ENTR U6 stuffer sgRNA scaffold L5-L4
Kanamycin
D / 11 pMuLE ENTR U6 stuffer sgRNA scaffold L5-L2
Kanamycin
D / 12 pMuLE ENTR U6 stuffer sgRNA scaffold R4-R3
Kanamycin
E / 1 pMuLE ENTR CMV-hCas9 R4-R3
Kanamycin
E / 2 pMuLE ENTR SV40-hCas9 L3-L2
Kanamycin
E / 3 pMuLE ENTR SV40-hCas9 L5-L2
Kanamycin
E / 4 pMuLE ENTR U6 n.s shRNA L1-R5
Kanamycin
E / 5 pMuLE ENTR U6 n.s shRNA L1-L4
Kanamycin
E / 6 pMuLE ENTR U6 n.s shRNA R4-R3
Kanamycin
E / 7 pMuLE ENTR U6 n.s shRNA L5-L4
Kanamycin
E / 8 pMuLE ENTR U6 n.s shRNA L5-L2
Kanamycin
E / 9 pMuLE ENTR CMV eGFP L5-L2
Kanamycin
E / 10 pMuLE ENTR CMV eGFP R4-R3
Kanamycin
E / 11 pMuLE ENTR CMV eGFP L1-R5
Kanamycin
E / 12 pMuLE ENTR CMV eGFP L3-L2
Kanamycin
F / 1 pMuLE ENTR SV40 eGFP L5-L2
Kanamycin
F / 2 pMuLE ENTR SV40 eGFP L3-L2
Kanamycin
F / 3 pMuLE ENTR CMV mCherry L5-L2
Kanamycin
F / 4 pMuLE ENTR CMV mCherry R4-R3
Kanamycin
F / 5 pMuLE ENTR CMV mCherry L1-R5
Kanamycin
F / 6 pMuLE ENTR SV40 mCherry L5-L2
Kanamycin
F / 7 pMuLE ENTR SV40 mCherry L3-L2
Kanamycin
F / 8 pMuLE ENTR CMV tdTomato R4-R3
Kanamycin
F / 9 pMuLE ENTR CMV tdTomato L5-L2
Kanamycin
F / 10 pMuLE ENTR SV40 tdTomato L3-L2
Kanamycin
F / 11 pMuLE ENTR SV40 tdTomato L5-L2
Kanamycin
F / 12 pMuLE ENTR SFFV tdTomato L5-L2
Kanamycin
G / 1 pMuLE ENTR CMV iRFP L1-R5
Kanamycin
G / 2 pMuLE ENTR CMV iRFP L5-L2
Kanamycin
G / 3 pMuLE ENTR CMV iRFP R4-R3
Kanamycin
G / 4 pMuLE ENTR CMV iRFP L3-L2
Kanamycin
G / 5 pMuLE ENTR SV40 iRFP L3-L2
Kanamycin
G / 6 pMuLE ENTR CMV PuroR L5-L2
Kanamycin
G / 7 pMuLE ENTR CMV PuroR R4-R3
Kanamycin
G / 8 pMuLE ENTR CMV PuroR L3-L2
Kanamycin
G / 9 pMuLE ENTR SV40 PuroR L3-L2
Kanamycin
G / 10 pMuLE ENTR SV40 PuroR L5-L2
Kanamycin
G / 11 pMuLE ENTR CMV LacZ L5-L2
Kanamycin
G / 12 pMuLE ENTR SV40 LacZ L5-L2
Kanamycin
H / 1 pMuLE ENTR CMV Luc2 (FF-Luciferase) L5-L2
Kanamycin
H / 2 pMuLE ENTR CMV Luc2 (FF-Luciferase) R4-R3
Kanamycin
H / 3 pMuLE ENTR SV40 Luc2 (FF-Luciferase) L5-L2
Kanamycin
H / 4 pMuLE ENTR SV40 Luc2 (FF-Luciferase) L3-L2
Kanamycin
H / 5 pMuLE ENTR CMV CreERT2 L5-L2
Kanamycin
H / 6 pMuLE ENTR CMV CreERT2 R4-R3
Kanamycin
H / 7 pMuLE ENTR SV40 CreERT2 L3-L2
Kanamycin
H / 8 pMuLE Lenti Dest eGFP
Chloramphenicol and Ampicillin
H / 9 pMuLE Lenti Dest iRFP
Chloramphenicol and Ampicillin
H / 10 pMuLE Lenti Dest IFP1.4
Chloramphenicol and Ampicillin
H / 11 pMuLE Lenti Dest Neo
Chloramphenicol and Ampicillin
H / 12 pMuLE Lenti Dest Luc2
Chloramphenicol and Ampicillin
Data calculated @ 2017-11-24

Kit Plate Map - #1000000060

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