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Reference Icon Redbook Chemogenetics Plasmids


Background

Chemogenetics is a technique that uses genetically engineered receptors to selectively control and investigate cell signalling pathways. Chemogenetic receptors are activated by small molecules, enabling remote control of signalling within cells expressing these engineered receptors. Chemogenetic receptors are popular in neuroscience, where they can be used to interrogate brain function and examine the relationship between neuronal signalling and behavior. These receptors also have broad applications in several fields and have been used to investigate a variety of cell types and mechanisms.

Chemoegenetics schematic

Schematic depicting the typical workflow of chemogenetics plasmid use in research

Chemogenetics Plasmids Available from Addgene

Addgene has a large collection of chemogenetics plasmids that can be used to interrogate cell function in various contexts. We also now offer ready-to-use AAV preparations of select chemogenetic plasmids, which can be used directly for in vivo or in vitro studies. Browse our list of chemogenetics plasmids below. Plasmids with the viral service icon are also offered as viral preparations.

Click on different properties to create a custom filtered list of the chemogenetics plasmids in Addgene's collection.

Promoter (localization)

Receptor

Plasmid type

Viral Service available 

ID Name Promoter Receptor Plasmid Type Activity PI
37825 pAAV-CAG-GFP CAG none AAV, Non-cre GFP expression control Boyden
59462 pAAV-CAG-tdTomato CAG none AAV, Non-cre tdTomato expression control Boyden
50469 pAAV-CaMKIIa-EGFP  CaMKIIa none AAV, Non-cre EGFP expression control Roth
50476 pAAV-CaMKIIa-hM3D(Gq)-mCherry  CaMKIIa hM3D(Gq) AAV, Non-cre Neuronal burst firing Roth
50477 pAAV-CaMKIIa-hM4D(Gi)-mCherry  CaMKIIa hM4D(Gi) AAV, Non-cre Neuronal silencing Roth
65418 pAAV-CaMKIIa-HA-KORD-IRES-mCitrine CaMKIIa KORD AAV, Non-cre Neuronal silencing Roth
50459 pAAV-hSyn-DIO-mCherry  Syn1 none AAV, Cre mCherry expression control Roth
44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry  Syn1 hM3D(Gq) AAV, Cre Neuronal burst firing Roth
44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry  Syn1 hM4D(Gi) AAV, Cre Neuronal silencing Roth
50458 pAAV-hSyn-DIO-rM3D(Gs)-mCherry Syn1 hM3D(Gs) AAV, Cre cAMP production Roth
50465 pAAV-hSyn-EGFP  Syn1 none AAV, Non-cre EGFP expression control Roth
50457 pAAV-hSyn-DIO-EGFP Syn1 none AAV, Cre EGFP expression control Roth
50474 pAAV-hSyn-hM3D(Gq)-mCherry  Syn1 hM3D(Gq) AAV, Non-cre Neuronal burst firing Roth
50475 pAAV-hSyn-hM4D(Gi)-mCherry  Syn1 hM4D(Gi) AAV, Non-cre Neuronal silencing Roth
65417 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine Syn1 KORD AAV, Cre Neuronal silencing Roth
50473 pAAV-GFAP-EGFP GFAP none AAV, Non-cre EGFP expression control Roth
58909 pAAV-GFAP104-mCherry GFAP none AAV, Non-cre mCherry expression control Boyden
50478 pAAV-GFAP-hM3D(Gq)-mCherry GFAP hM3D(Gq) AAV, Non-cre Neuronal burst firing Roth
50479 pAAV-GFAP-hM4D(Gi)-mCherry GFAP hM4D(Gi) AAV, Non-cre Neuronal silencing Roth
75032 pAAV CD68-hM3D(Gq)-mCherry CD68 hM3D(Gq) AAV, Non-cre Neuronal burst firing Roth
75033 pAAV CD68-hM4D(Gi)-mCherry CD68 hM4D(Gi) AAV, Non-cre Neuronal silencing Roth
75034 pAAV CD68-EGFP CD68 none AAV, Non-cre EGFP expression control Roth
50454 pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine Syn1 hM3D(Gq) AAV, Cre Neuronal burst firing, cell filling Roth
50455 pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine Syn1 hM4D(Gi) AAV, Cre Neuronal silencing, cell filling Roth
50456 pAAV-hSyn-DIO-HA-hM3D(Gs)-IRES-mCitrine Syn1 hM3D(Gs) AAV, Cre cAMP production, cell filling Roth
50460 pAAV-EF1a-DIO-hM3D(Gq)-mCherry EF1a hM3D(Gq) AAV, Cre Neuronal burst firing Roth
50461 pAAV-EF1a-DIO-hM4D(Gi)-mCherry EF1a hM4D(Gi) AAV, Cre Neuronal silencing Roth
50462 pAAV-EF1a-DIO-mCherry EF1a none AAV, Cre mCherry expression control Roth
50463 pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine Syn1 hM3D(Gq) AAV, Non-cre Neuronal burst firing, cell filling Roth
50464 pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine Syn1 hM4D(Gi) AAV, Non-cre Neuronal silencing, cell filling Roth
50466 pAAV-CaMKIIa-HA-hM3D(Gq)-IRES-mCitrine CaMKIIa hM3D(Gq) AAV, Non-cre Neuronal burst firing, cell filling Roth
50467 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine CaMKIIa hM4D(Gi) AAV, Non-cre Neuronal silencing, cell filling Roth
50468 pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine CaMKIIa rM3D(Gs) AAV, Non-cre cAMP production, cell filling Roth
50470 pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine GFAP hM3D(Gq) AAV, Non-cre Neuronal burst firing, cell filling Roth
50471 pAAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine GFAP hM4D(Gi) AAV, Non-cre Neuronal silencing, cell filling Roth
50472 pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine GFAP rM3D(Gs) AAV, Non-cre cAMP production, cell filling Roth
45547 pcDNA5/FRT-HA-hM3D(Gq) CMV hM3D(Gq) Mammalian expression Inositol monophosphate (IP1) accumulation, ERK1/2 phosophorylation Roth
45548 pcDNA5/FRT-HA-hM4D(Gi) CMV hM4D(Gi) Mammalian expression Neuronal silencing Roth
45549 pcDNA5/FRT-HA-rM3D(Gs) CMV rM3D(Gs) Mammalian expression Roth
70717 AAV-mOXT-hM3Dq-mCherry-WPRE oxytocin hM3D(Gq) AAV, Non-cre Geschwind
66795 pAAV-PTRE-tight-hM3Dq-mCherry tet-inducible hM3D(Gq) AAV, Non-cre Wisden
81008 pAAV-CBA-DIO-hM4Di-mCherry CBA hM4D(Gi) AAV, Cre Sabatini
24429 pcDNA3-Rog CMV RASSL Mammalian expression Conklin
24503 pcDNA3-Ro2 CMV RASSL Mammalian expression Conklin
24504 pcDNA3-Ro1 CMV RASSL Mammalian expression Conklin
16312 pUNIV-SIG-5HTR4D100A h5HT4b Mammalian expression Conklin
16313 pUHG10.3-TetO-Rs1 TetO h5HT4b Mammalian expression Conklin

Currently, no available plasmids.

Browse all Chemogenetics Plasmids

G Protein Chimera Plasmids

G protein chimeras can be used to alter the signalling phenotype of G-protein coupled receptors (GPCRs). These chimeras have been used to study receptor signalling, ligand binding, effector coupling, and other molecular mechanisms of GPCRs. For a user manual on these plasmids, see the supplemental document or user manual created by the depositor.

ID Name Gene Mutation Depositor Comments PI
24502 q4wt Gq alpha none This is Gq alpha with an HA epitope engineered into an internal site that does not seem to affect receptor coupling in multiple studies Conklin
24498 qs5 Gq alpha Replacement of the five carboxyl-terminal amino acids of Gq alpha with those of Gs alpha This construct allows some Gs-coupled receptors to stimulate phospholipase C Conklin
24501 qi5 Gq alpha Gq alpha with the C-terminal amino acids changed from Gq alpha to Gi alpha residues This construct allows many Gi-coupled receptors to stimulate phospholipase C Conklin
24500 qo5 Gq alpha Gq alpha with the C-terminal amino acids changed from Gq alpha to Go alpha residues Works the same as qi5 but (for unknown reasons) has a slightly lower basal PLC activity Conklin
25867 qz5 Gq alpha Replacement of the five carboxyl-terminal amino acids of Gq alpha with those of Gz alpha Works the same as qi5 Conklin
24499 sq5 Gs alpha This is Gs alpha with the C-terminal amino acids changed from Gs alpha to Gq alpha residues This construct allows some Gq-coupled receptors to stimulate Adenylate cyclase Conklin