pAAV-EF1a-DIO-hM4D(Gi)-mCherry
(Plasmid #50461)

• Purpose: Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoter
• Depositing Lab: Bryan Roth
• Publication: Roth lab DREADDs (unpublished)

Backbone

• Vector backbone: pAAV
  (Search Vector Database)
• Backbone size w/o insert (bp): 4818
• Total vector size (bp): 7772
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: hM4D(Gi)-mCherry
• Species: H. sapiens (human)
• Insert Size (bp): 2175
• Mutation: See supplemental documents for DREADD mutations
• Entrez Gene: CHRM4 (a.k.a. HM4, M4R)
• Promoter: EF1a
• Tags / Fusion Proteins:
  • HA (N terminal on insert)
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Asc I (not destroyed)
• 3′ cloning site: Nhe I (not destroyed)
• 5′ sequencing primer: TTTTTGAGTTTGGATCTTGG
• 3′ sequencing primer: GCATTAAAGCAGCGTATCCACATAGC

Resource Information

• Supplemental Documents:
  • DREADD mutations
• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 11 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry