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pAAV-EF1a-DIO-hM4D(Gi)-mCherry
(Plasmid #50461)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50461 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 4818
  • Total vector size (bp) 7772
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    hM4D(Gi)-mCherry
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2175
  • Mutation
    See supplemental documents for DREADD mutations
  • Entrez Gene
    CHRM4 (a.k.a. HM4, M4R)
  • Promoter EF1a
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Asc I (not destroyed)
  • 3′ cloning site Nhe I (not destroyed)
  • 5′ sequencing primer TTTTTGAGTTTGGATCTTGG
  • 3′ sequencing primer GCATTAAAGCAGCGTATCCACATAGC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1a-DIO-hM4D(Gi)-mCherry was a gift from Bryan Roth (Addgene plasmid # 50461 ; http://n2t.net/addgene:50461 ; RRID:Addgene_50461)