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protocol icon Addgene Videos

Browse Addgene's collection of video content. Find video protocols, tips on using Addgene's resources, and career advice. For written lab protocol material, visit our Addgene Protocols page. Please feel free to email us at [email protected] with any questions.

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Video Link Description Related Page
Making LB Agar Plates Create plates to culture bacteria in the lab Pouring LB Agar Plates Protocol
Streaking Bacteria on Plates Isolate single bacterial colonies on an agar plate Streaking Bacteria on Plates Protocol
Diagnostic Restriction Digest Verify a plasmid using restriction enzymes Diagnostic Restriction Digest Protocol
Storing and Handling Addgene Plasmids What to do after you receive your plasmids from Addgene Instructions for Storing and Handling Plasmids
Genomic Deletions with CRISPR Generate genomic deletions in mammalian cells lines using CRISPR/Cas9 Genomic Deletions with CRISPR Protocol
Over-Agar Antibiotic Plating Create plates to culture bacteria in the lab Over-Agar Antibiotic Plating Protocol
AAV Purification Purify adeno-associated virus from a preparation produced in mammalian cells AAV Purification by Iodixanol Gradient Ultracentrifugation Protocol
Mulitchannel Pipetting Technique Learn how to pipette multiple samples at once using a multichannel pipette and avoid inconsistent pipetting with our simple tips
Bacterial Transformations We walk you through a standard plasmid transformation protocol and offer some tips and tricks to reduce transformation time and increase efficiency Bacterial Transformation Protocol
Inoculating a Liquid Bacterial Culture How to prepare and grow bacteria in a liquid medium Inoculating a Liquid Bacterial Culture Protocol

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How-To Videos

Video Link Description Related Page
MTA Guide - Requests Your guide to the MTA process when requesting samples from Addgene Technology Transfer
How to Order A step-by-step walkthrough of Addgene's ordering process Ordering Page
MTA FAQs Answers to frequently asked questions about the MTA process Help Center
Browse and Search Tips and tricks for browsing and searching Addgene's expansive plasmid collection Addgene's Collection
How to Deposit Your guide to depositing plasmids with Addgene Depositing Page

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Career Videos

Video Link Description
Jessica Welch, PhD In this first installment of the Addgene Careers series, we sit down with Addgene Outreach Scientist Jessica Welch to discuss her career path, differences in research culture between the US and Australia, and advice for current graduate students considering their future careers.
Eric J. Perkins, PhD In this installment, we sit down with Senior Scientific Project Leader Eric Perkins to discuss the various positions he has held in his career, his favorite company tradition, and Addgene's role in the scientific community.
Jane Hannon Our Marketing & Outreach Specialist Jane Hannon discusses her career path, current projects in the works, and all of the things she loves about working at Addgene!
Maria Soriano Maria Soriano, from Addgene's UK office, discusses her career in science, the importance of international collaboration, and offers career advice for scientists looking to expand their international reach.
Joel McDade Joel McDade, Addgene's Business Development Manager, discusses his transition from the Scientists Team to Business Team, his hobbies, and advice for grauated students pursuing non-academic careers.