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viral-icon-01.png Viral Service: Lentivirus


Addgene's lentiviruses are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive a ready-to-use viral preparation from the inventory below.

Lentiviruses are distributed as purified viral particles in solution that are suitable for in vitro use. Volume and minimum titers are listed on each item's material page. Actual titers are reported with each shipment.

For more details about our viral service, visit our Viral Service landing page.

Browse Available Lentivirus

Screen.png Pooled CRISPR Libraries for Screening

Use pooled CRISPR libraries to screen for genes involved in specific biological processes. For more information, see our Guide to Plasmid Pooled Libraries.

ID Name Description Genes/Insert Selection PI
Human knockout pooled libraries
73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone does not contain SpCas9. 76,441 unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin Doench, Root
73179 Human gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin Doench, Root
Human activation pooled libraries
92379
New!
Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) targeting 18,885 genes and containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting 18,885 human genes Puromycin Doench, Root
92380
New!
Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set B Human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) targeting 18,843 genes and containing 56,476 unique sgRNAs. This backbone does not contain dCas9. 56,476 unique sgRNAs targeting 18,843 human genes Puromycin Doench, Root
Mouse knockout pooled libraries
73633 Mouse sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls 78,637 unique sgRNAs targeting 19,674 mouse genes along with 1000 non-targeting controls Puromycin Doench, Root

Cut.png Cas9 Lentivirus

Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins can be guided to specific genomic sites by an RNA guide (gRNA) that harbors targeting sequences. See our list of experimentally validated gRNA viruses in viral format below or browse our repository for a list of validated gRNA plasmids. For more information on CRISPR plasmids and function, see our CRISPR plasmids resource page.

Browse our list of available Cas9 viruses by function:

Cut.png  Cas9 for Editing Genomic Sequences

To perform genome edits, Cas9 nuclease is used to generate double-stranded breaks (DSBs or cuts) or single-stranded breaks (nicks).

ID Name Insert Function Antibiotic Resistance Activity PI
52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. Lentiviral backbone. Zhang
63593 lentiCas9n (D10A)-Blast Sp Cas9 Nick Blasticidin Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone. Zhang

Activate.png  Cas9 and Accessories for Activating Gene Expression

Catalytically-dead Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional activator.

ID Name Insert Antibiotic Resistance Activity PI
61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A GFP Zhang
61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang
61426 lenti MS2-P65-HSF1_Hygro MS2-P65-HSF1 Hygromycin lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE) Zhang

Interfere.png  Cas9 for Repressing Gene Expression

When fused to a transcriptional repressor, catalytically-dead Cas9 mutants (dCas9) can interfere with and reduce transcription.

ID Name Antibiotic Resistance Activity PI
50919 pHAGE EF1α dCas9-KRAB Puromycin Constitutive dCas9-KRAB lentiviral expression vector Maehr, Wolfe

Visualize.png  Cas9 for Visualizing Genomic Loci

When fused to a fluorescent protein, catalytically-dead Cas9 (dCas9) can be used to visualize genomic loci.

ID Name Antibiotic Resistance Activity PI
64107 pHAGE-TO-dCas9-3XGFP none Expression of Sp dCas9-3XGFP in mammalian cells Pederson
64108 pHAGE-TO-dCas9-3XmCherry none Expression of Sp dCas9-3XmCherry in mammalian cells Pederson

Epigenetics.png  Cas9 for Editing DNA Methylation

Fusion of catalytically-dead Cas9 (dCas9) to epigenetic modifiers enables targeted DNA methylation editing. By the addition or removal of methyl groups to a promoter sequence, gene expression can be silenced or activated, respectively.

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gRNA.png gRNA Lentivirus

Browse our list of experimentally validated gRNA viruses to target specific genes. These viruses are for use in Cas9-expressing cells. For more gRNA plasmids, browse our list of validated gRNA plasmids and use our protocols to generate lentivirus.

ID Name Target Antibiotic Resistance Purpose PI
59702 pXPR_011 EGFP Puromycin This lentiviral vector can be used to assay Cas9 activity Doench, Root
76479 BRAF gRNA (BRDN0001145345) BRAF Puromycin 3rd generation lentiviral gRNA plasmid targeting human BRAF Doench, Root
76480 BRAF gRNA (BRDN0001146266) BRAF Puromycin 3rd generation lentiviral gRNA plasmid targeting human BRAF Doench, Root
76510 MAP3K4 gRNA (BRDN0001148639) MAP3K4 Puromycin 3rd generation lentiviral gRNA plasmid targeting human MAP3K4 Doench, Root
80034 EGFP gRNA (BRDN0000561167) EGFP Puromycin 3rd generation lentiviral gRNA plasmid targeting EGFP Doench, Root
80035 EGFP gRNA (BRDN0000562805) EGFP Puromycin 3rd generation lentiviral gRNA plasmid targeting EGFP Doench, Root
80036 EGFP gRNA (BRDN0000563266) EGFP Puromycin 3rd generation lentiviral gRNA plasmid targeting EGFP Doench, Root

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lentiviral-icon.png Non-CRISPR Lentivirus

Browse our list of available viruses based on plasmids in our repository that are not used for CRISPR.

ID Name Insert Antibiotic Resistance Purpose PI
1864 scramble shRNA scramble Puromycin 3rd gen lentiviral negative control vector containing scrambled shRNA Sabatini
12265 pHR-CMV-nlsCRE Cre none CMV-driven expression of Cre recombinase Trono
17408 Puro.Cre empty vector Cre Puromycin Empty backbone expressing Cre recombinase and pyromycin resistance Jacks
17446 pLenti CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau
59700 pRosetta EGFP Puromycin, Blasticidin Lentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions Doench, Root
74218 pLenti puro HA-Ubiquitin ubiquitin Puromycin General lentiviral expression plasmid Fan
83842 pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 none Fluorescent probe for S/G2 cell cycle transition and G2/M transition Lin

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Don’t See What You’re Looking For?

Please let us know what you would like to see available in viral format using the form below. Please note this does not guarantee viral service, but lets us know what viruses would be helpful to the scientific community.