Viral Service: Lentivirus
We are offering ready-to-use viral preparations of several popular lentiviral plasmids and pooled libraries in our repository. With viral service, Addgene provides high-quality and high-titer viral preparations. We perform quality control experiments, including using our lentiviruses for stable cell line generation, colony formation titering assay, or fluorescence titering assay. You can see the results of our quality control experiments on the material pages for viral catalog items. For descriptions of Addgene's viral production protocols and quality controls, visit our viral production page. For protocols on how to use virus from Addgene or how to make your own virus, see our list of viral protocols with tips and sample data.
Addgene's lentiviruses are made from plasmids that are currently in our repository, so feel free to obtain the plasmid from us, or take advantage of our viral service by browsing our available lentiviruses below!
Each viral service request will also include virus associated DNA, which is a sample of the purified DNA from the original plasmid that was used to make the virus. In order for Addgene to provide viral services to you, we must ensure that you have the right to use the original plasmid.
Distribution of the original plasmid is important because it is the actual material being referenced in the Material Transfer Agreement (MTA) and its inclusion is what gives you permission to use the plasmid.
Browse Available Lentivirus by Function
Lentiviral Preparations of Pooled CRISPR Libraries for Screening
Use pooled CRISPR libraries to screen for genes involved in specific biological processes. For more information, see our Guide to Plasmid Pooled Libraries.
|73178||Human sgRNA library Brunello in lentiGuide-Puro||Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls||76,441 unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls||Puromycin||Root|
|73633||Mouse sgRNA library Brie in lentiGuide-Puro||Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls||78,637 unique sgRNAs targeting 19,674 mouse genes along with 1000 non-targeting controls||Puromycin||Root|
Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins can be guided to specific genomic sites by an RNA guide (gRNA) that harbors targeting sequences. See our list of experimentally validated gRNA viruses in viral format below or browse our repository for a list of validated gRNA plasmids. For more information on CRISPR plasmids and function, see our CRISPR plasmids resource page.
Browse our list of available Cas9 viruses by function:
Cas9 for Editing Genomic Sequences
|52962||lentiCas9-Blast||Sp Cas9||Cut||Blasticidin||Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.||Zhang|
|63593||lentiCas9n (D10A)-Blast||Sp Cas9||Nick||Blasticidin||Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.||Zhang|
Cas9 and tools for Activating Gene Expression
Catalytically-dead Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional activator.
|61422||dCAS9-VP64_GFP||dCAS9 (D10A, H840A)||none||Expresses dCAS9-VP64 activator with 2A GFP||Zhang|
|61425||lenti dCAS-VP64_Blast||dCAS9 (D10A, N863A)||Blasticidin||3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)||Zhang|
|61426||lenti MS2-P65-HSF1_Hygro||MS2-P65-HSF1||Hygromycin||lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE)||Zhang|
Cas9 for Repressing Gene Expression
When fused to a transcriptional repressor, catalytically-dead Cas9 mutants (dCas9) can interfere with and reduce transcription.
|50917||pHAGE TRE dCas9-KRAB||Neomycin (select with G418)||Tet-regulatable dCas9-KRAB lentiviral expression vector||Maehr, Wolfe|
|50919||pHAGE EF1α dCas9-KRAB||Puromycin||Constitutive dCas9-KRAB lentiviral expression vector||Maehr, Wolfe|
Cas9 for Visualizing Genomic Loci
When fused to a fluorescent protein, catalytically-dead Cas9 (dCas9) can be used to visualize genomic loci.
|64107||pHAGE-TO-dCas9-3XGFP||none||Expression of Sp dCas9-3XGFP in mammalian cells||Pederson|
|64108||pHAGE-TO-dCas9-3XmCherry||none||Expression of Sp dCas9-3XmCherry in mammalian cells||Pederson|
Cas9 for Editing DNA Methylation Coming soon!
Fusion of catalytically-dead Cas9 (dCas9) to epigenetic modifiers enables targeted DNA methylation editing. By the addition or removal of methyl groups to a promoter sequence, gene expression can be silenced or activated, respectively.
|84475||Fuw-dCas9-Tet1CD||Coming Soon||DNA demethylation by the enzymatic domain of Tet1||Jaenisch|
|84479||Fuw-dCas9-Tet1CD_IM||Coming Soon||Control: inactive catalytic domain of Tet1 (H1672Y, D1674A)||Jaenisch|
|84476||Fuw-dCas9-Dnmt3a||Coming Soon||DNA methylation by de novo DNA methyltransferase Dnmt3a||Jaenisch|
|84478||Fuw-dCas9-Dnmt3a_IM||Coming Soon||Control: inactive Dnmt3a catalytic domain (E664A, E756A)||Jaenisch|
Browse our list of experimentally validated gRNA viruses to target specific genes. These viruses are for use in Cas9-expressing cells. For more gRNA plasmids, browse our list of validated gRNA plasmids and use our protocols to generate lentivirus.
|59702||pXPR_011||EGFP||Puromycin||This lentiviral vector can be used to assay Cas9 activity||Doench, Root|
|76479||BRAF gRNA (BRDN0001145345)||BRAF||Puromycin||3rd generation lentiviral gRNA plasmid targeting human BRAF||Doench, Root|
|76480||BRAF gRNA (BRDN0001146266)||BRAF||Puromycin||3rd generation lentiviral gRNA plasmid targeting human BRAF||Doench, Root|
|76510||MAP3K4 gRNA (BRDN0001148639)||MAP3K4||Puromycin||3rd generation lentiviral gRNA plasmid targeting human MAP3K4||Doench, Root|
|80034||EGFP gRNA (BRDN0000561167)||EGFP||Puromycin||3rd generation lentiviral gRNA plasmid targeting EGFP||Doench, Root|
|80035||EGFP gRNA (BRDN0000562805)||EGFP||Puromycin||3rd generation lentiviral gRNA plasmid targeting EGFP||Doench, Root|
|80036||EGFP gRNA (BRDN0000563266)||EGFP||Puromycin||3rd generation lentiviral gRNA plasmid targeting EGFP||Doench, Root|
Browse our list of available viruses based on plasmids in our repository that are not used for CRISPR.
|1864||scramble shRNA||scramble||Puromycin||3rd gen lentiviral negative control vector containing scrambled shRNA||Sabatini|
|12265||pHR-CMV-nlsCRE||Cre||none||CMV-driven expression of Cre recombinase||Trono|
|17408||Puro.Cre empty vector||Cre||Puromycin||Empty backbone expressing Cre recombinase and pyromycin resistance||Jacks|
|17446||pLenti CMV GFP Hygro (656-4)||GFP||Hygromycin||3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro||Campeau|
|26429||pLenti CMV rtTA3 Blast (w756-1)||Tetracycline repressor A3 mutant||Blasticidin||3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast||Campeau|
|59700||pRosetta||EGFP||Puromycin, Blasticidin||Lentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions||Doench, Root|
|74218||pLenti puro HA-Ubiquitin||ubiquitin||Puromycin||General lentiviral expression plasmid||Fan|
Don’t See What You’re Looking For?
Please let us know what you would like to see available in viral format using the form below. Please note this does not guarantee viral service, but lets us know what viruses would be helpful to the scientific community.